布洛芬对创伤小鼠细胞免疫功能的影响

目的 选用前列腺素合成抑制剂布洛芬（Ｉｂｕ）对创伤后细胞免疫功能变化的作用。方法Ｂａｂｌ／ｃ小鼠行创伤性截肢手术后分为Ｉｂｕ组（腹腔注射Ｉｂｕ１ｍｇ／ｋｇ，ｎ＝２８）和生理盐水组（腹腔注射生理盐水０．５ｍｌ，ｎ＝２８），正常小鼠作为对组。手术后１、３、５、７天分别每组取７只小鼠测其淋巴细胞增殖功能和自然杀伤细胞（ＮＫ细胞）活性。结果 创伤后小鼠脾脏淋巴细胞增殖功能明显下降（Ｐ〈０．０１），于第７天

Effect of ibuprofen on cellular immunity of mice after trauma

Objective To evaluate the effect of cyclooxygenase inhibitor ibuprofen,on the cellular immunity of mice after trauma.Methods The study was conducted in Balb/c mice(6 8weeks old)with one of their legs amputated.After the operation,the mice were allocated to receiving ibuprofen 1mg/kg ip(Ibu group) or normal saline(NS group).The normal mice served as control(control group).Then 1,3,5,7days after the operation,seven mice of each group were sacrificed to assess their splenocyte proliferation and NK cell cytotoxicity.Results Splenocyte proliferation was depressed markedly in the NS group(P<0.01 vs control group) and had not recovered 7 days later (P<0.05 vs control group).Splenocyte proliferation above in Ibu group was much lower than control group during the 1st and 3rd day (P<0.01),but recovered on the 5th day(P>0.05) and was higher than NS group(P<0.01).The percent of NK cell cytotoxicity of Ibu group was also higher than of NS group(1st day P<0.01,3rd day P<0.05),lower than of control group (P<0.01).Conclusion The depressed cellular immunity following trauma can be partially reversed by cyclooxygenase inhibitor,indicating that prostaglandin may play an important role in the trauma induced immunosuppression.