蜂胶对牙龈卟啉单胞菌及牙龈成纤维细胞的影响

目的了解蜂胶对牙龈卟啉单胞菌（porphyromonas gingival，Pg）生长的抑制作用及对人牙龈成纤维细胞的毒性作用。方法采用液体稀释法测定蜂胶对Pg的最小杀菌浓度（MBC），用MTT法测定不同浓度蜂胶作用24h后牙龈成纤维细胞的细胞相对增殖率（RGR），了解蜂胶作用24h后细胞连续7d的增殖回复情况。结果蜂胶对取的MBC为1．56g／L，相当于0．125～0．25mg／L的奥硝唑。蜂胶浓度为MBC时RGR达到70％以上；4g／L蜂胶组的RGR为52．1％，作用细胞24h后，连续7d细胞数持续增加，吸光度逐渐接近阴性对照组。结论蜂胶对Pg的生长有显著抑制作用，且细胞毒性较小，毒性作用可逆。

Effect of propolis on porphyromonas gingival and human gingival fibroblast

Objective To evaluate the effects of propolis on the growth of porphyromonas gingival(Pg)and its toxical influence on human gingival fibroblast(HGF).Methods Minimal bactericidal concentration(MBC)of propolis on Pg was determined by the fluid dilution method.The relative growth rates(RGR)and the proliferation status were determined by MTT assay after HGF was incubated in culture solution containing propolis for 24 h.Results MBC of propolis to Pg was 1.56 g/L,which was equal to 0.125 to 0.25 mg/L ornidazole.T...