| 药用植物决明的愈伤组织诱导与生长增值的培养条件研究 |
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| 引用本文： | 王镨,宋家祥,付群英.药用植物决明的愈伤组织诱导与生长增值的培养条件研究[J].中草药,1997,28(12):739-742. |
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| 作者姓名： | 王镨 宋家祥 付群英 |
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| 作者单位： | 浙江工业大学轻工系 杭州310004;浙江工业大学轻工系 杭州310004;浙江工业大学轻工系 杭州310004 |
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| 摘 要： | 本实验成功地以决明的胚轴、子叶诱导出愈伤组织。实验表明，ＭＳ比Ｂ５更利于愈伤组织诱导，正交试验表明，添加１ｍｇ／Ｌ的ＩＡＡ、６－ＢＡ、ＧＡ３可明显促进愈伤组织的生长；添加１ｍｇ／Ｌ的ＮＡＡ、２，４－Ｄ可抑制其生长；添加１ｍｇ／Ｌ的６－ＢＡ、２，４－Ｄ对抑制愈伤组织的分化有明显的效应。适合愈伤组织生长及维持分化率低水平蔗糖浓度为２－３％。愈伤组织的诱导及生长周期为３０天左右。
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| 关 键 词： | 药用作物 决明 愈伤组织 组织培养 |
Studies on Induction and Growth of Caiiuses of Jueming(Cassia abtusifolia) |
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| Wang Pu,Song Jiaxiang.Studies on Induction and Growth of Caiiuses of Jueming(Cassia abtusifolia)[J].Chinese Traditional and Herbal Drugs,1997,28(12):739-742. |
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| Authors: | Wang Pu Song Jiaxiang |
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| Institution: | Department of Light Industry , Zhejiang University of Technology , HangZhou;Department of Light Industry , Zhejiang University of Technology , HangZhou;Department of Light Industry , Zhejiang University of Technology , HangZhou |
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| Abstract: | Calluses of Cassia abtusifolia I. were accessfully induced in different media. Calluses were induced betier in IVIS than in 135. Adding lmg/I1/1.A, lmyil. G-13A and lmg/IGA3 could improve the noticeably. However,Nr A and 2.4) could depress the growth of calluses.6-BA and 2 growth of calluses 4-D showed apparent depressive effects on the differentiation of calluses.Suitable concentration of sucrose for improving the growth of calluses and lowering the differentiation of calluses was 2%-3%. The growth cycle of calluses was about 30 days. |
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