甘薯长喙壳菌毒素检测的高效薄层色谱法研究

用乙醚提取甘薯毒素 ,提取物分别用 5 %的Na2CO3 和蒸馏水洗涤 ,无水硫酸钠干燥 ,蒸去乙醚。残渣 (粗品 )采用高效薄层色谱法进行检测 ,以纯品甘薯酮和甘薯酮醇作标样。结果表明 :在 2 0℃条件下在石油醚 醋酸乙酯 (2∶1 ,v v)溶剂系统中 ,对甘薯酮 (C)、甘薯酮醇 (A)等毒素有较好的分离效果 ,Rf 值的重复性也较好。展开后的薄层板通过CS 930薄层扫描仪在 52 0nm下扫描定量。采用高效薄层色谱法检测甘薯酮及甘薯酮醇 ,最低检测限量 :纯品为 0 0 0 2 μg,粗品为 0 0 1 μg。

Study of ceratocystis fimbriata toxins determination from sweet potato by high performance thin-layer chromatography

The extract solution from Sweet Potato was washed with 5% Na2CO3 and followed with distilled water and dried with dehydrated sodium sulfate (Na2SO4). The ether extract was evaporated and the residue (crude sample) was dissolved in a small volume of chloroform-soluble solution and was spotted on a silica gel G thin-layer plate developed with different solvent systems. Pure ipomeamarone and ipomeamaronol were used as standards. The resolution was the best in the solvent system of petroleum ether-ethylacetate at 20 degrees C. The separated spots were scanned by CS-930 TLC scanner at 520 nm and quantitative determination by external standard calibration. Ipomeamarone was detected by high performance thin-layer chromatography and the limits of detection were 0.002 microgram for pure and 0.01 microgram for crude sample.