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| 室内氡暴露居民肺癌p53和k-ras基因突变 | |
| 引用本文： | 夏英,杨梅英,吕慧敏,冯治孝,叶常青,王作元.室内氡暴露居民肺癌p53和k-ras基因突变[J].中华放射医学与防护杂志,2001,21(6):404-405. |
| 作者姓名： | 夏英 杨梅英 吕慧敏 冯治孝 叶常青 王作元 |
| 作者单位： | 1. 卫生部工业卫生实验所, 2. 北京放射医学研究所 3. 甘肃庆阳地区医院 |
| 基金项目： | 国家自然科学基金资助项目(39970235) |
| 摘 要： | 目的：检测室内高氡暴露地区居民肺癌P53和k-ras基因突变，方法：采用PCR扩增，PCR－SSCP电泳和序列分析方法。结果：9例标本中有5例p53PCR-SSCP电泳呈阳性结果，其中3例经克隆测序发现有碱基置换，结论：室内氡浓度在200－350Bq.m^-3.a^-1范围内的7例肺癌病例中有5例发生P53基因突变，P53基因突变还可能与肺癌患者的年龄吸烟史有关。 |
| 关 键 词： | 氡 肺癌 p53 PCR－SSCP 序列分析 |
| 收稿时间： | 2001/4/17 0:00:00 |
| 修稿时间： | 2001年4月17日 |
p53 and k-ras mutations in lung cancer associated with residential radon exposure |
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| XIA Ying,YANG Meiying,L&#; Huimin.p53 and k-ras mutations in lung cancer associated with residential radon exposure[J].Chinese Journal of Radiological Medicine and Protection,2001,21(6):404-405. | |
| Authors: | XIA Ying YANG Meiying L&#; Huimin |
| Institution: | XIA Ying,YANG Meiying,L Huimin,et al.Laboratory of Industrial Hygiene,Ministry of Health,Beijing 100088,China |
| Abstract: | Objective The aim of this study was to analyze mutation of p53 and k-ras genes in lung cancer related to residential radon exposure. Methods PCR amplification,PCR-SSCP electrophoresis,and sequence analysis were carried out. Results Five of 9 lung cancer cases were found to be positive with p53 PCR-SSCP electrophoresis techniques,and in 3 among the 5 base substitution was observed. Conclusion Five of the seven cases of lung cancer 200-350 Bq·m-3 ·a-1 radon level had p53 gene mutation,which might be related to age and smoking status of the patients. |
| Keywords: | Radon \ Lung cancer \ p53 \ PCR\|SSCP \ Sequence analysis |
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