| Difference of Gene Expression Profiles between Barrett＇s Esophagus and Cardia Intestinal Metaplasia by Gene Chip |
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| 引用本文： | 常英,刘斌.Difference of Gene Expression Profiles between Barrett＇s Esophagus and Cardia Intestinal Metaplasia by Gene Chip[J].华中科技大学学报(医学英德文版),2006,26(3):311-313. |
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| 作者姓名： | 常英 刘斌 |
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| 作者单位： | [1]Gastroenterology Endoscopic Department, Sixth Hospital of Shanghai Jiaotong University, Yishan Road, Shanghai 200233, China [2]Department of Emergence Surgery, Shaanxi Provincial People＇s Hospital, Xian 710068, China |
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| 摘 要： | The difference of gene expression profile changes in Barrett＇s esophagus （BE） and cardia intestinal metaplasia （CIM） epithelium was studied and the novel associated genes were screened in the early stage by cDNA microarray. The cDNA retro-transcribed from equal quantity mRNA from BE and CIM epithelial tissues were labeled with Cy3 and Cy5 fluorescence as probes. The mixed probe was hybridized with three pieces BiostarH-40s double dot human whole gene chip. The chips were scanned with a ScanArray 4000. The acquired images were analyzed using GenePix Pro 3.0 software. It was found a total of 141 genes were screened out that exhibited differentially expression more than 2 times in all three chips. It was identified that in gene expression profiles of BE, 74 genes were up-regulated and 67 down-regulated as compared with CIM. The comparison between the difference of gene expression profile changes in BE and CIM epithelia revealed that there existed the difference between BE and CIM at gene level. 141 genes with the expression more than two time were probably related to the occurrence and development of BE and the promotion or progress in adenocarcinoma.
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| 关 键 词： | 基因表达 食道疾病 肠化生 病理机制 |
| 收稿时间： | 2005-12-22 |
Difference of gene expression profiles between Barrett’s esophagus and cardia intestinal metaplasia by gene chip |
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| Chang Ying,Liu Bin.Difference of gene expression profiles between Barrett’s esophagus and cardia intestinal metaplasia by gene chip[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2006,26(3):311-313. |
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| Authors: | Chang Ying Liu Bin |
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| Institution: | 1. Gastroenterology Endoscopic Department, Sixth Hospital of Shanghai Jiaotong University, Yishan Road, Shanghai 200233, China
2. Department of Emergence Surgery, Shaanxi Provincial People's Hospital, Xi'an 710068, China |
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| Abstract: | Summary The difference of gene expression profile changes in Barrett’s esophagus (BE) and cardia intestinal metaplasia (CIM) epithelium
was studied and the novel associated genes were screened in the carly stage by cDNA microarray. The cDNA retro-transcribed
from equal quantity mRNA from BE and CIM epithelial tissues were labeled with Cy3 and Cy5 fluorescence as probes. The mixed
probe was hybridized with three pieces BiostarH-40s double dot human whole gene chip. The chips were scanned with a ScanArray
4000. The acquired images were analyzed using GenePix Pro 3.0 software. It was found a total of 141 genes were screened out
that exhibited differentially expression more than 2 times in all three chips. It was identified that in gene expression profiles
of BE, 74 genes were up-regulated and 67 down-regulated as compared with CIM. The comparison between the difference of gene
expression profile changes in BE and CIM epithelia revealed that there existed the difference between BE and CIM at gene level.
141 genes with the expression more than two time were probably related to the occurrence and development of BE and the promotion
or progress in adenocarcinoma.
CHANG Ying, female, born in 1969, Associate Professor, M. D. |
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| Keywords: | Barrett's esophagus cardia intestinal metaplasia gene expression profile |
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