| 微波协助提取在中药饮片含量测定中的应用(6) ——微波法与药典法测定野菊花中蒙花苷含量比较 |
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| 引用本文： | 方婧,杨洪军,付梅红,安琪,张贝贝,王祝举,杨岚,张东.微波协助提取在中药饮片含量测定中的应用(6) ——微波法与药典法测定野菊花中蒙花苷含量比较[J].中国实验方剂学杂志,2012,18(24):94-96. |
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| 作者姓名： | 方婧 杨洪军 付梅红 安琪 张贝贝 王祝举 杨岚 张东 |
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| 作者单位： | 1. 中国中医科学院中药研究所,北京,100700
2. 首都医科大学,北京,100069 |
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| 基金项目： | "重大新药创制"科技重大专项(2009ZX09308-003);"国家科技支撑计划子课题"(2006BAI09B07-02) |
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| 摘 要： | 目的:建立微波协助提取法提取野菊花中有效成分蒙花苷含量测定方法,比较微波协助提取法与药典法提取蒙花苷优势。方法:采用Dikma Diamonsil C18色谱柱(4.6 mm×150 mm,5μm),流动相甲醇-水-冰醋酸(26∶23∶1),检测波长334nm,柱温30℃,流速1 mL.min-1。结果:微波提取时间15 min,提取温度100℃,提取溶剂50%乙醇。蒙花苷在0.04～1.2μg呈良好线性关系(r=0.999 9),平均回收率100.13%(n=6)。结论:微波提取法较药典法提取蒙花苷更简便快速、准确,为一种符合环保绿色理念的含量测定方法,可用于检测野菊花饮片中蒙花苷含量。
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| 关 键 词： | 微波提取 野菊花 蒙花苷 含量测定 |
| 收稿时间： | 2011/5/10 0:00:00 |
Determination of Microwave-Assisted Extraction of Chinese Cut Crude Drugs(6) Comparison of the Determination of Linarin from Chrysanthemum indicum Between Microwave-Assisted Extraction and Chinese Pharmacopeia Method |
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| FANG Jing,YANG Hong-jun,FU Mei-hong,AN Qi,ZHANG Bei-bei,WANG Zhu-ju,YANG Lan and ZHANG Dong.Determination of Microwave-Assisted Extraction of Chinese Cut Crude Drugs(6) Comparison of the Determination of Linarin from Chrysanthemum indicum Between Microwave-Assisted Extraction and Chinese Pharmacopeia Method[J].China Journal of Experimental Traditional Medical Formulae,2012,18(24):94-96. |
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| Authors: | FANG Jing YANG Hong-jun FU Mei-hong AN Qi ZHANG Bei-bei WANG Zhu-ju YANG Lan and ZHANG Dong |
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| Institution: | Institute of Chinses Materia Medica,China Academy of Traditional Chinese Medicine, Beijing 100700,China;Institute of Chinses Materia Medica,China Academy of Traditional Chinese Medicine, Beijing 100700,China;Institute of Chinses Materia Medica,China Academy of Traditional Chinese Medicine, Beijing 100700,China;Capital Medical University,Beijing 100069,China;Institute of Chinses Materia Medica,China Academy of Traditional Chinese Medicine, Beijing 100700,China;Institute of Chinses Materia Medica,China Academy of Traditional Chinese Medicine, Beijing 100700,China;Institute of Chinses Materia Medica,China Academy of Traditional Chinese Medicine, Beijing 100700,China;Institute of Chinses Materia Medica,China Academy of Traditional Chinese Medicine, Beijing 100700,China |
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| Abstract: | |
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| Keywords: | microwave-assisted extraction Chrysanthemum indicum linarin determination |
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