不同型号大孔树脂对金荞麦中有效成分的纯化工艺考察

目的:考察不同型号大孔树脂对金荞麦中有效成分的纯化效果。方法:以表儿茶素和金E的含量、浸膏率为指标,结合HPLC指纹图谱,选择最佳型号大孔树脂。结果:AB-8型大孔树脂对表儿茶素洗脱率为89.78%,对金E洗脱率为93.04%。HPLC指纹图谱共有峰面积比较表明AB-8型大孔树脂最适合用于金荞麦有效成分的纯化。结论:采用AB-8型大孔树脂纯化金荞麦中有效成分是可行的,且操作简单、稳定。

Investigation of Purification Technology for Effective Constituents from Fagopyrum dibotrys by Different Types of Macroporous Resin

Objective:To investigate purification effect of different types of macroporous resin on effective constituents from Fagopyrum dibotrys. Method: With the content of epicatechin and mixture of proanthocyanidins tannic condensation,extract rate as indexes,optimum resin was selected by combining with HPLC fingerprint. Result: Elution rate of epicatechin by AB-8 resin was 89.78%,elution rate of mixture of proanthocyanidins tannic condensation by AB-8 resin was 93.04%.Comparison of the common peak area of HPLC fingerprint showed that AB-8 resin was the most suitable one to purify effective constituents from F. dibotrys. Conclusion: This process of applying AB-8 resin to purity effective constituents from F. dibotrys was feasible, simple and stable and could be extended to large-scale production applications.