高孜万药材中总黄酮含量的测定

目的:测定几种不同来源的维吾尔药材高孜万中总黄酮含量,为高孜万药材的筛选及推广提供技术支撑。方法:以芦丁为对照品,采用亚硝酸钠-硝酸铝-氢氧化钠显色法,在510 nm处利用紫外分光光度法测定意大利牛舌草、琉璃苣、糙草、倒提壶等4种不同来源的高孜万药材中总黄酮的含量。结果:意大利牛舌草、琉璃苣、倒提壶、糙草等4种不同来源的高孜万中总黄酮的含量分别为意大利牛舌草3.85 mg.g-1、琉璃苣3.72 mg.g-1、倒提壶2.35 mg.g-1、糙草(和田)3.14 mg.g-1、糙草(策勒)4.73 mg.g-1、糙草(于田)3.76 mg.g-1。其中策勒产糙草中总黄酮含量最高,倒提壶含量最低。结论:该方法准确、可靠、重复性好,可以用作高孜万中总黄酮含量的测定。糙草、琉璃苣和意大利牛舌草中总黄酮的含量没有太大的差别。

Determination of Total Flavonoids in Several Gaoziban

Objective:To determine the content of total flavonoids in several different sources of Gaoziban. Method: Using rutin as a reference substance, the content of total flavonoids in Gaoziban of Asperugo procumbens from Hetian, Cele, and Yutian, Anchusa italica,Borage officinales, and Cynoglossum officinales were determined at 510 nm by UV spectrophotometry colored with sodium nitrite-aluminum nitrate-sodium hydroxide. Result: Content of total flavonoids in three Gaozibans of Anchusa italica, Borage officinales, Cynoglossum officinales were 3.85, 3.72, 2.35 mg·g^-1; and three Gaozibans of A. procumbens from Hetian, Cele and Yutian were 3.14, 4.73, 3.67 mg·g^-1 respectively, among which the content of total flavonoids in Gaoziban was highest in A. procumbents from Cele and lowest in C. officinales. Conclusion: The method was accurate, reliable, repeatable, and could be used to determine the content of total flavonoids in Gaoziban. The content of total flavonoids is not much different from A. italica, B. officinales and A. procumbens.