Basigin mRNA在早期缺血心肌和非缺血心肌的表达变化

目的 探讨Basigin mRNA在早期心肌缺血大鼠模型缺血心肌(EIM)和非缺血心肌(NIM)中的表达及意义.方法 以β-actin为内对照,应用real-time PCR方法 检测0 min、15 min、30 min、60 min早期缺血心肌和非缺血心肌Basigin mRNA的表达情况.结果 EIM、NIM、假手术(S0)组心肌和非手术组心肌Basigin mRNA表达在0 min差异没有统计学意义(P＞0.05).EIM组在心肌缺血15 min、30 min和60 min较0 min时Basigin mRNA表达降低,差异有统计学意义(P＜0.001),NIM组和SO组在不同时间段之间差异无统计学意义(P＞0.05);EIM组和NIM组在30 min、60 min时Basigin mRNA表达的差异有统计学意义(P＜0.05).结论 Basigin在缺血30 min参与心肌的病理生理过程,Basigin在鉴定早期心肌缺血死亡的案例中有十分重要的法医学意义.

The Expression of Basigin mRNA in Early Ischemic Myocardium and Non-ischemic Myocardium

Objective To investigate the expression of Basigin mRNA in early ischemic myocardium(EIM)and non-ischemic myocardium(NIM)in rat.Methods Real-time polymerase chain reaction(PCR)technique was applied for detecting Basigin mRNA andβ-actin expression in EIM and NIM at 0 rain,15 min,30 min,and 60 min.Results No significant differences of the Basigin mRNA expression in EIM and NIM between 0 min and sham operation(SO)or non-operation group(P＞0.05)were observed.Basigin mRNA expression in EIM significantly decreased in 15 min,30 min,and 60 min group compared with that of 0 min group,respectively(P＜0.001).However,we failed to find any significant difference in NIM and NO from 0 min to 60 min.Basigin mRNA expression in EIM decreased significantly compared with that of in NIM(P＜0.05)after myocardial ischemia for 30 min.Conclusion Basigin was involved in myocardium pathophysiology process after myocardial ischemia for 30 min,indicating that Basigin may be identified as a predictor of early myocardial ischemia in forensic medicine.