多西紫杉醇对鼠血管肉瘤细胞株的体外抑制作用

目的 探讨多西紫杉醇（Docetaxel，TXT）对鼠血管肉瘤细胞株的增殖抑制作用。方法 采用Alamar Blue法测定，TXT对ISOS-1细胞增殖抑制的影响，并与其它抗肿瘤药物进行比较；用流式细胞仪测定细胞周期的变化。结果 TXT能显著抑制ISOS-1细胞的增殖，IC50为15．8ng／ml，明显低于其它抗肿瘤药物；TXT作用后细胞周期发生明显变化，高浓度TXT对ISOS-1细胞株有G2-M期阻滞作用，低浓度TXT主要将ISOS-1细胞阻断于C0-G1期并诱导细胞凋亡。结论 TXT能强烈抑制鼠血管肉瘤细胞株ISOS-1的增殖，这种抑制作用可能通过作用G2-M期及诱导细胞凋亡来实现的。

The inhibiting effect of TXT on murine angiosarcoma cell line in vitro

Objective To study the proliferation inhibition effect of TXT on murine angiesarcoma cell line. Methods To determinate the proliferation inhibition effect of TXT on ISOS-1 cells by Alamar Blue assay, and compare TXT with other chemodrugs. The changes of cell cycle was determinated by FCM. Results TXT can inhibit the proliferation of ISOS- 1 cells obviously, the IC50 was 15. 8 ng/ml, which was lower than the rates of other chemodrugs. Cell cycles changed much after TXT was applied. High concentration TXT can block ISOS- 1 cell line at G2-M phase, while low concentration TXT can block ISOS- 1 cells at G0-G1 phase to induce apoptosis.Condusion TXT can inhibit the proliferation of murine angiosarcoma cell line ISOS-1 strongly, it can not only act on G2-M phase, but also induce apoptesis.