大孔树脂分离纯化玉米苗提取物中提取物中薏苡素的研究

目的研究不同型号大孔树脂纯化玉米苗提取物中薏苡素的工艺条件及参数。方法以静态饱和吸附量、洗脱量、静态洗脱率为参考指标,比较了8种大孔树脂,并对LX-11树脂的动态比上柱量,比吸附量进行了考察,以薏苡素转移率和纯度为指标对树脂吸附工艺条件进行了筛选。结果 8种树脂中LX-11树脂具有最佳的吸附及洗脱参数,其最佳工艺是浓度为0.155 4～0.233 2 mg.mL-1的上样液,以3倍柱体积蒸馏水,5倍70%乙醇依次洗脱,薏苡素转移率为88%左右,纯度为9%左右。结论 LX-11树脂综合性能较好,适合玉米苗提取物中薏苡素的纯化。 更多还原

Separation and purification of 6-MBOA from fresh maize seedling by macroreticular resin

Objective To investigate the technology for purifying and separation of total favanoids from fresh maize seedling with different types of macroporous resin（MR）.Methods The static capacity adsorption and static elution ratio of 8 types of MR were studied respectively and compared.The dynamic saturation ratio,dynamic adsorption ratio of LX-11 and the yields,and purification degree of 6-MBOA were compared to obtain the optimum technological parameters.Results LX-11 type of MR showed optimum adsoption and elution parameters.The concentration of fresh maize seedling extract was 0.155 4-0.233 2 mg· mL-1.After being eluted with 3 column volume of ditillde water and 5 column volume of 70% ethanol,the yield of 6-MBOA was 88% and the product purification was 9%.Conclusion LX-11 type of MR shows good comprehensive adsorption,and it may be used to purify the 6-MBOA.