| Rg_2与R_f对钙通道作用的单通道分析 |
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| 摘 要： | 在单个Ｗｉｓｔａｒ大鼠乳鼠心室肌细胞上，用连细胞电压钳法，观察人参三醇组皂甙单体Ｒｇ２、Ｒｆ６０μｍｏｌ·Ｌ－１对钙通道单通道活动的影响，并与钙通道阻滞剂异博定７９μｍｏｌ·Ｌ－１、钙通道激动剂ＢＡＫ８６４４５μｍｏｌ·Ｌ－１对照，证明Ｒｇ２对钙通道有阻滞作用，其机理在于使钙通道的开放时间缩短、开放概率减少、关闭时间延长，而对Ｂａａ＋流幅值无明显影响，Ｒｆ可缩短Ｌ型钙通道的开放时间、延长关闭时间、降低开放概率。
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| 关 键 词： | 钙通道，人参皂甙，斑片钳，BAYK8644 |
Action Analysis of Rg2、Rf on calcium channel |
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| Authors: | Zhang Wenjie Liu Wei Zhan Shu et
al |
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| Institution: | Department of Physiology; Central Labolaboratory of Physiology |
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| Abstract: | The influence of
panaxatrio1 saponin monomers Rg2、Rf 60 μmol·L-1 on single Calci-um channel activity
from cultured neonatal Wistar rat myocardiocyte was observed using the cell-at-tached
configuration of patch clamp technique,and compared with that of calcium channel blocker
ver-apamil 79 μmol·L-1 and calcium channel activator BAY K 8644 5μmol·L-1. The
inhibiting effectsof Rg2 on the three-type calcium channels were observed. The mechanism
was that Rg2 shortented theopen time ,decreased the open一state probability, lengthened the
close time of calcium channels,with-out influencing the amplitude of the ionic current flowing
through the opening calcium channel;Rf hadno these actions. |
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| Keywords: | Ca2+ channel ginsenosides patch clamp BAY K 8644 |
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