微量ELISA技术在检测森脑病人特异IgM抗体中的应用

本文报告用间接微量酶联免疫吸附试验检测森脑特异IgM抗体。在37份采自病后不同时期的森脑患者血清中,共检出25例阳性。试验发现森脑特异IgM在病后1周内即以较高滴度存在,2周左右达到高峰,然后迅速下降,至5月后基本消失。未发现血清中同时存在的特异IgG对IgM检测有明显影响。通过对类风湿因子阳性血清的检测、SPA吸收试验、阻断试验、酶抗体结合物试验等,证实了检测结果的可靠性。这一方法简单、敏感、特异,适用于森脑的早期诊断。

The use of an indirect micro-ELISA for detection of IgM antibodies against RSSE

An indirect micro ELISA was developed for detection of IgM antibodies against RSSE virus. Of 37 sera from RSSE patients, 25 showed positive results. It is found that RSSE-specific IgM was detectable in high titre as early as 4 days after onset of the disease, reached its peak in the second week, then dropped down rapidly and disappeared within 5 months. The phenomenon that the xistence of specific IgG may affect the detection of specific IgM is not found. The specificity of this technique was confirmed by detection of RF-positive sera, blocking test and SPA absorbing test. It is suggests that this ELISA system can be used as a simple,rapid and sensitive method for early diagnosis of RSSE.