IL-12诱导类风湿关节炎和骨关节炎外周血T淋巴细胞STAT4活化状态的比较

目的：比较类风湿关节炎和骨关节炎外周血T淋巴细胞在IL12作用下诱导STAT4酪氨酸磷酸化水平的差异。方法：2007年5月至2009年8月分离提纯20例类风湿关节炎患者均为女性;年龄28～55岁,平均（45.0±13.0）岁]和20例骨关节炎患者均为女性;年龄55～75岁,平均（67.0±9.6）岁]的外周血T淋巴细胞,流式细胞仪进行T淋巴细胞纯度鉴定。用50ng/mlIL12分别作用0、10、30、60min后,提取蛋白进行浓度测量,然后进行WesternBlot检测,经蛋白条带灰度扫描,比较IL12作用各时间点类风湿关节炎和骨关节炎T淋巴细胞STAT4酪氨酸磷酸化水平的差异。结果：经过对外周血单核细胞的分离纯化,T细胞纯度达到91%以上;IL12在对骨关节炎和类风湿关节炎T细胞作用30min后达到活化峰值,在各个时间点（10、30、60min）类风湿关节炎外周血T淋巴细胞活化水平均高于骨关节炎。结论：类风湿关节炎外周血T淋巴细胞在IL12作用下STAT4异常活化。

Comparison of the status of STAT4 tyrosine phosphorylation in peripheral T-lymphocytes induced by IL-12 in rheumatoid arthritis and osteoarthritis

Objective: To compare the levels of STAT4 tyrosine phosphorylation in peripheral T-lymphocytes induced by IL-12 in rheumatoid arthritis(RA) and osteoarthritis(OA). Methods: From May 2007 to August 2009, peripheral blood mononuclear cells(PBMCs) were isolated from RA patientsRA group, all the cases were female, the age was from 28 to 55 years with an average of (45.0±13.0) years] and OA patientsOA group, all the cases also were female;the age was from 55 to 75 years with an average of (67.0±9.6) years]. The purity of T-lymphocytes from PBMCs was accredited by flow cytometry. The IL-12 of 50 ng/ml added in T-lymphocytes, the levels of STAT4 tyrosine phosphorylation were detected by western blot after different time intervals(0, 10, 30, 60 min). Results: The purity of T-lymphocytes were above 91% through diremption and depuration for peripheral blood monouclear cells. The levels of STAT4 tyrosine phosphorylation in T-lymphocytes from RA induced by IL-12 were higher than that from OA in the different times(10, 30, 60 min);after 30 min, its levels from RA and OA achieved to crest value. Conclusion: STAT4 in peripheral T-lymphocytes of rheumatoid arthritis was more easily to be activated than osteoarthritis.