骨碎补总黄酮对去卵巢大鼠骨超微结构及脯氨酸羟化程度的影响

目的：研究应用骨碎补总黄酮后骨超微结构及脯氨酸羟化程度的变化,从骨胶原的超微结构来研究骨碎补总黄酮对骨强度的影响。方法：40只10月龄SD雌性大鼠（体重350～380g）,随机分成假切除组（空白组）、模型组、药物组、对照组,每组10只。假切除组只切除腹部的少许脂肪,其余各组采用双侧卵巢切除的方法进行动物造模。药物组每天灌服中药骨碎补总黄酮浓缩剂2ml（相当于骨碎补总黄酮0.02g）,共6个月;对照组每天灌服培美力悬剂2ml（相当于倍美力0.017mg）,共6个月。灌胃6个月后取L2进行扫描电镜观察,并以碱水解法测单位重量脯氨酸羟化程度。结果：骨碎补总黄酮能明显改善骨胶原的排列,减少断裂的骨胶原。药物组脯氨酸羟化程度为（58.77±0.56）%,对照组为（60.90±0.53）%,模型组为（47.34±0.52）%,药物组和对照组比较差异无统计学意义,但较模型组脯氨酸羟化程度提高（P〈0.05）。结论：骨碎补总黄酮能改善松质骨的超微结构及脯氨酸羟化程度,从而为药物防治骨质疏松的研究提供思路。

Effects of drynaria fortunei on bone ultramicrostructure and proline hydroxylation level of ovariectomized rats

Objective: To observe the change of bone ultramicrostructure and proline hydroxylation level after application of drynaria fortunei and explore the effects of drynaria fortunei to bone strength from aspect of ultramicrostructur of bone collagen. Methods: Forty SD female rats with 10-month-old(Each weight was 350-380 g) were randomly divided into sham-ovariectomized group,model group,Chinese herb group and control group(each group had 10 rats). Only a little abdominal fat were cut in sham-ovariectomized group;in other groups,the hibateral ovaries of rats were cut to make ostmenopausal osteoporasis model. Chinese herb medicine group rats were treated with drynaria fortunei of 2 ml (equivalent to 0.02 g) through intragastric administration everyday,and control group were fed with Premarin of 2 ml (equivalent to 0.017 mg). At the 6 months after treatment,bone ultramicrostructure of L₂ were observed with electronmicroscope,and proline hydroxylation level were measured by method of alkali hydrolysis. Results: Drynaria fortunei can obviously impove arrangement of bone collagen and decrease break of bone collegen. Proline hydroxylation level of Chinese herb medicine group,control group,model group were respectively(58.77±0.56)%,(60.90±0.53)%,(47.34±0.52)%,there was no significant difference between Chinese herb medicine group and control group,but this two groups were higher than that of model group (P<0.05). Conclusion: The experimental results showed that drynaria fortunei can improve cancellated bone ultramicrostructure and proline hydroxylation level,thereby to provide a new idea for prevention and treatment of osteoporosis.