92例非霍奇金淋巴瘤组织中Skp2和p27kip1蛋白的表达及相互关系

目的探讨92例非霍奇金淋巴瘤（NHL）组织中Skp2和p27^kip1蛋白的表达及相互关系。方法采用免疫组织化学方法检测92例NHL和14例反应性增生淋巴结组织中Skp2、p27^kip1蛋白及细胞增殖指标Ki-67的表达情况,结合病理资料进行统计分析;采用Western blot技术检测4种淋巴瘤细胞株内Skp2和p27^kip1蛋白的表达情况。结果Skp2蛋白在NHL组织中的表达高于反应性增生的淋巴结（不含生发中心）,且与增殖活性（Ki-67 LI）呈正相关,并随着肿瘤侵袭性增加而表达增高;p27^kip1蛋白在NHL组织中的表达低于反应性增生淋巴结（不含生发中心）,且与增殖活性呈负相关,并随着肿瘤侵袭性增加而表达减低;Skp2和p27^kip1蛋白在NHL组织中的表达无明显相关性。结论在NHL组织中,Skp2的高表达和p27^kip1的低表达均可能与NHL的发生发展有关。

Expression and correlation of Skp2 and p27(kip1) in 92 cases of non-Hodgkin's lymphoma

Objective To investigate the expression and correlation of Skp2 and p27kip1 in non-Hodgkin's lymphoma. Methods The expression of Skp2, p27kip1 and Ki-67(the proliferation index) were detected in sections of 92 cases of non-Hodgkin's lymphoma(NHL)and 14 cases of reactive lymph nodes by immunohistochemistry and histopathology. The expression of Skp2 and p27kip1 in 4 NHL cell lines were detected by Western blot. Results The expression of Skp2 in NHL cases were significantly higher than that in reactive lymph nodes (except the germinal centers) , positively correlated with proliferation activity, and an increasing tumor aggressiveness was associated with the increased expression of Skp2. The expression of p27kip1 protein in NHL cases were significantly lower than that in reactive lymph nodes (except the germinal centers), negatively correlated with proliferation activity, and an increasing tumor aggressiveness was associated with decreased expression of p27kip1. The statistical analysis indicated that there was no obvious correlation between Skp2 and p27kip1 expression in NHL tissues. Conclusion The higher expression of Skp2 and lower expression of p27kip1 in NHL tissues may play a role in the tumorigenesis and development of NHL.