谷胱甘肽硫转移酶基因多态性与儿童获得性再生障碍性贫血易感性关系的初步研究

目的 探讨谷胱甘肽硫转移酶(Glutathione S transferases,GST)基因多态性与儿童再生障碍性贫血(AA)易感性的关系.方法 选择本院2005年6月-2008年6月确诊的42例初诊AA患儿,对照组选择68例保健门诊查体儿童,分别抽取骨髓及外周血2 mL,用淋巴细胞分离液提取单个核细胞,储存于-49...

Study on the relationship between genetic polymorphisms of glutathione S transferases T1, M1 genes and susceptibility of children with acquired aplastic anemia

Objective To explore the potential relationship between genetic polymorphisms of glutathione reductase-S transferases T1,M1 genes and the susceptibility of children with acquired aplastic anemia in Shanxi Province.Methods Forty-two children with acquired aplastic anemia and 68 normal controls treated in Xi′an Children′s Hospital from June of 2005 to June of 2008 were enrolled into this study.The genetic polymorphisms of GSTT1 and GSTM1 genotypes were detected by multiplex polymerase chain reaction.Chi-square analysis was used to compare the rate of gene deletion between patient and control groups.Odds ratio(OR) and 95% of confidence interval(CI) were analyzed meanwhile.Results There were no significant differences of gene deletion rate between patient and control groups.Conclusion The gene deletion rate in children with acquired aplastic anemia appeared to be lower than the control,though there were no statistical differences between the two groups.The susceptibility of children with GSTT1 and GSTM1 deletion might decreased,which needed further validation by more samples.