重组RA538,反义c—myc腺病毒对bcl—2高表达细胞系的作用及分子 …

目的 研究重组ＲＡ５３８（Ａｄ－ＲＡ５３８）、反义ｃ－ｍｙｃ腺病毒（Ａｄ－ＡＳｃ－ｍｙｃ）在ｂｃｌ－２高表达细胞系中的作用及其分析机制。方法 采用细胞形态学观察、ＭＴＴ、ＲＴ－ＰＣＲ和Ｎｏｒｔｈｅｒｎ ｂｌｏｔ等方法，研究ＲＡ５３８，反义ｃ０ｍｙｃ重组腺病毒在ｂｃｌ－２高表达的人宫颈癌细胞系ＨｅＬａ－ｂｃｌ２和ＳｉＨａ－ｂｃｌ２以及在其亲本细胞中的生物学作用及其分子机制。结果 以ｌｉｐｏｆｅｃｔｉ

The effects of RA538 and antisense c myc on cervical cancer cell lines with high expression of bcl-2 gene

Objective To study the effects of recombinant adenovirus with RA538 or antisense c myc insert on cervical cancer cell lines with high expression of bcl 2 gene.Methods Cervical cancer cell lines HeLa and SiHa were transduced with full length bcl 2 cDNA by lipofectin and cell clones with stable expression of bcl 2 were selected. They were then transfected with recombinant adenovirus containing RA538 (Ad RA538), antisense c myc (Ad ASc myc) or LacZ (Ad LacZ) gene. Morphologic and molecular changes of the transfected cancer cells were examined by light microscopy, MTT, RT PCR and Northern blot. Results The bcl 2 cDNA was successfully transferred into HeLa and SiHa cells. Two cell lines, called HeLa bcl2 and SiHa bcl2 with high expression of bcl 2 gene were generated. Ad RA538 and Ad ASc myc transfection both could inhibit cell growth and induce apoptosis of HeLa and SiHa cells, and inhibit their expression of c myc and bcl 2 genes. However, although Ad RA538 and Ad ASc myc had some inhibitory effect on bcl 2 and c myc gene expression by HeLa bcl2 and SiHa bcl2 cells, they could only slightly inhibit cell growth and weakly induce apoptosis.Conclusion Ad RA538 and Ad ASc myc can to certain extent inhibit cell growth and induce apoptosis of two cervical cancer cell lines. They have little such effect when these cell lines have over expressed bcl 2 by transduction of exogenous bcl 2 gene.