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| HPLC-MS/MS测定大鼠血浆中丹参素钠的浓度 | |
| 引用本文： | 王晶,金磊,熊礼燕,王庭芳,郑莉萍,张宁,邹豪,张川. HPLC-MS/MS测定大鼠血浆中丹参素钠的浓度[J]. 药学实践杂志, 2011, 29(2): 109-110,130 |
| 作者姓名： | 王晶 金磊 熊礼燕 王庭芳 郑莉萍 张宁 邹豪 张川 |
| 作者单位： | 1. 第二军医大学药学院新药研究中心,上海,200433 2. 第二军医大学药学院新药研究中心,上海,200433;福建中医药大学药学院,福建,福州,350108 3. 上海市徐汇区中心医院,上海,200031 |
| 摘 要： | 目的建立液质联用色谱法（HPLC-MS/MS）测定SD大鼠血浆中丹参素钠的浓度。方法血浆样品采用液液萃取方法进行预处理。色谱柱为Diamonil C18柱（4.6mm×200mm,5μm）;流动相为甲醇-水（含0.01‰甲酸）=（80：20）;流速为1.0ml/min;质谱条件：采用ESI离子源,负离子检测,选择MRM测定丹参素钠和酮洛芬197→135m/z和253→209m/z。结果丹参素钠在10.6～1060ng/ml检测浓度范围内呈良好的线性关系（r=0.9996）,最低定量限为10.6ng/ml,日内和日间精密度的RSD〈7.8%,回收率在99%到102%之间。结论该方法灵敏度高、快速、简单、准确,适用于血浆样品中丹参素钠的含量测定以及临床前实验研究 |
| 关 键 词： | 丹参素钠 HPLC-MS／MS 血药浓度 |
| 收稿时间： | 2011-03-01 |
| 修稿时间： | 2011-03-07 |
Determination of sodium Danshensu in rat plasma by HPLC-MS/MS |
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| WANG Jing,JIN Lei,XIONG Li-yan,WANG Ting-fang,ZHENG Li-ping,ZHANG Ning,ZOU Hao and ZHANG Chuan. Determination of sodium Danshensu in rat plasma by HPLC-MS/MS[J]. The Journal of Pharmaceutical Practice, 2011, 29(2): 109-110,130 | |
| Authors: | WANG Jing JIN Lei XIONG Li-yan WANG Ting-fang ZHENG Li-ping ZHANG Ning ZOU Hao ZHANG Chuan |
| Affiliation: | New drug research center, School of Pharmcy, Second military medical university, Shanghai 200433, China;New drug research center, School of Pharmcy, Second military medical university, Shanghai 200433, China;New drug research center, School of Pharmcy, Second military medical university, Shanghai 200433, China;New drug research center, School of Pharmcy, Second military medical university, Shanghai 200433, China;School of Pharmacy, Fujian university of traditional Chinese medcine , Fuzhou 350108, China;New drug research center, School of Pharmcy, Second military medical university, Shanghai 200433, China;School of Pharmacy, Fujian university of traditional Chinese medcine , Fuzhou 350108, China;Central Hospital of Xuhui District, Shanghai 200031, China;New drug research center, School of Pharmcy, Second military medical university, Shanghai 200433, China;New drug research center, School of Pharmcy, Second military medical university, Shanghai 200433, China |
| Abstract: | Objective To estiblish a method of determining sodium Danshensu in rat plasma by HPLC-MS/MS.Methods sodium Danshensu was extrated for determination by HPLC-MS/MS.Analytical column was Diamonsil C18（4.6 mm×200 mm,5μm）.The mobile phase was methanol：water（0.01‰formic acid ） = 80：20.Mass spectrum conditions were ESI performing in the MRM mode using target ions m/z 197→135（Sodium Danshensu）,m/z 253→209（keloprofen）.Results The calibration curve was liner over the range of 10.6～1 060 ng/ml.The LLOQ ol sodium Danshensu in plasma was 10.6 ng/ml.The intra-day and inter-day RSD were〈7.8%.The extracted recovery was ranged from 99%to 102%.Conclusion The method was sensitive,rapid,simple and accurate to sodium Danshensu plasma concertration and suitable to study preclinical test of sodium Danshensu. |
| Keywords: | sodium Danshensu HPLC-MS/MS plamsma concentration |
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