不同氧浓度下大鼠肝细胞有氧代谢酶CCO、PDH、KGDH活性的实验研究

目的：观察缺氧对肝脏实质细胞糖有氧氧化的影响。方法：利用体外培养的肝细胞缺氧模型，模拟创伤后的缺血，缺氧状态，以正常培养的大量肝细胞为对照，采用生物化学的方法。分析不同氧浓度及氧缺时间下肝细胞氧氧化关键酶活性的变化，并测定了细胞内ATP含量的变化。结果：与正常对照组相比，缺氧肝细胞内丙酮酸脱氢酶（PDH）、α－酮戊二酮脱氢酶（KGDH）、细胞色素C氧化酶（CCO）活性在缺氧培养1h即显著降低，并持续到16h，缺氧时肝细胞ATP含量随时间延长而下降，4h达到最低。结论：缺氧后肝细胞有氧氧化及细胞内ATP的产生严重受抑。

Changes of activities of CCO, PDH, KGDH in aerobic metabolism in rat BRL cells after hypoxia

Objective To observe the effects of hypoxia caused by trauma on glucose aerobic metabolism in cultured rat liver cells. Methods Cells were exposed to different concentrations of oxygen to simulate hypoxic state after trauma, and then the activities of enzymes related to glucose aerobic metabolism in hepatocytes were determined. Hepatocytes cultured under normal condition were used as normal control. Results Compared with those of the normal control group, the activities of PDH, KGDH, CCO were significantly lower under different hypoxic conditions, especially in 1% O 2 group. Conclusion Glucose aerobic metabolism and production of ATP are inhibited by hypoxia.