| 纳米磁性探针转染大鼠外周血内皮祖细胞的磁共振成像实验研究 |
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| 引用本文： | 曹爱红,石红建,聂芳,杨芳,滕皋军. 纳米磁性探针转染大鼠外周血内皮祖细胞的磁共振成像实验研究[J]. 第三军医大学学报, 2008, 30(20): 1948-1951 |
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| 作者姓名： | 曹爱红 石红建 聂芳 杨芳 滕皋军 |
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| 作者单位： | 东南大学,附属中大医院放射科,江苏省分子影像与功能影像实验室,南京,210009;徐州医学院第二附属医院放射科,江苏,徐州,221006;东南大学,附属中大医院放射科,江苏省分子影像与功能影像实验室,南京,210009;东南大学,生物科学与医学工程系,南京,210009 |
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| 摘 要： | 目的 建立稳定的体外分离培养、诱导分化大鼠外周血内皮祖细胞(endothelial progenitor cells, EPCs),及应用纳米磁探针体外标记EPCs的方法,磁共振(MR)进行标记细胞成像.方法 抽取SD大鼠外周血,应用Ficoll密度梯度离心的方法获得单个核细胞,同时应用VEGF和bFGF 诱导,使之向内皮细胞分化,以免疫细胞化学鉴定贴壁细胞的内皮标志.制备Fe2O3-arginine复合物,对EPCs进行磁探针标记,应用MR进行细胞群成像.结果 经VEGF和bFGF诱导后的EPCs的内皮标志CD31、CD34、Flk21和vWF在不同时段呈阳性表达.普鲁士蓝染色可见铁颗粒位于细胞质内,标记率接近100%;磁共振成像(MRI)显示标记的细胞群信号强度的变化较未标记细胞群信号降低.结论 大鼠外周血中含有EPCs,在体外特殊诱导环境下,可定向分化为内皮样细胞.Fe2O3-arginine可以有效标记EPCs构建纳米生物探针,临床应用型1.5T MR可在体外进行标记细胞群成像.
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| 关 键 词： | 内皮祖细胞 纳米生物探针 内皮细胞 分化 离体 标记 磁共振成像 |
Magnetic resonance imaging of endothelial progenitor cells from rat peripheral blood labeled with nano-bioprobe of superparamagnetic iron oxide |
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| CAO Ai-hong,SHI Hong-jian,NIE Fang,YANG Fang,TENG Gao-jun. Magnetic resonance imaging of endothelial progenitor cells from rat peripheral blood labeled with nano-bioprobe of superparamagnetic iron oxide[J]. Acta Academiae Medicinae Militaris Tertiae, 2008, 30(20): 1948-1951 |
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| Authors: | CAO Ai-hong SHI Hong-jian NIE Fang YANG Fang TENG Gao-jun |
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| Affiliation: | CAO Ai-hong1,2,SHI Hong-jian1,NIE Fang1,YANG Fang3,TENG Gao-jun1(1Department of Radiology,Jiangsu Key Laboratory of Molecular Imaging , Functional Imaging,Zhong Da Hospital,3School of Biological Sciences , Medical Engineering,Southeast University,Nanjing 210009,2Department of Radiology,The Second Hospital of Xuzhou Medical College,Xuzhou Jiangsu 210006,China) |
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| Abstract: | Objective To investigate the isolation,culture,proliferation and differentiation of endothelial progenitor cells(EPCs) from rat circulating blood and to study of magnetic resonance imaging of nano-bioprobe that was constructed with endothelial progenitor cells and superparamagnetic iron oxide(SPIO).Methods The mononuclear cells(MNCs) were isolated from SD rat peripheral blood,and EPCs were selected by adherence method.Then the expressions of cell markers of EPCs were assessed by immunohistochemistry.EPCs we... |
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| Keywords: | endothelial progenitor cells nano-bioprobe endothelial cells differentiation isolation labeling magnetic resonance imaging |
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