吲哚硒对小鼠化学性肝损伤的保护作用

目的 探讨吲哚硒对四氯化碳(CCl4)致小鼠急性化学性肝损伤的保护作用及可能机制。 方法 建立CCl4诱导小鼠化学性肝损伤模型，分光光度法检测血浆中ALT、AST水平和肝匀浆MDA、SOD、GSH-Px含量，HE染色法对肝脏组织作病理检查。结果 吲哚硒(5、10、20mg／kg)灌胃给药均能降低血浆中升高的转氨酶水平。病理检查也发现其可明显减轻肝组织坏死范围及程度，减少炎细胞浸润。同时发现吲哚硒可降低肝匀浆中升高的MDA水平，使降低的肝匀浆SOD、GSH-Px酶活性升高。结论 吲哚硒对CCl4致小鼠急性化学性肝损伤具有保护作用。其机制与其抗自由基、提高抗氧化物酶的活性等有关。

Protective effects of indole-selenium of indole selenium on chemical liver injury induced in mice

Objective To investigate the effects of indole-selenium on carbon tetrachloride-induced liver injury. Methods The model of mice chemical liver injury was prepared.The level of alanine aminotransferase(ALT), aspartate aminotransferase(AST) in plasma, malondiadehyde(MDA) content, superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activities in liver homogenate were assayed by spectrophotometer; Hepatic pathological examination was observed. Results Indole-selenium (5,10,20 mg/kg) was able to significantly decrease serum transaminase (ALT,AST) levels, attenuate the area and extent of necrosis and reduce the infiltration of inflammatory cells. Furthermore, indole-selenium also decreased MDA content and improved the reduced SOD and GSH-Px level in liver homogenate. Conclusion Indole-selenium possesses protective action on chemical liver injury in mice.