Specificity of IS6110-based DNA fingerprinting and diagnostic techniques for Mycobacterium tuberculosis complex |
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| Authors: | Githui W A Wilson S M Drobniewski F A |
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| Affiliation: | PHLS Mycobacterium Reference Unit, Dulwich Public Health Laboratory and Department of Microbiology, Kings College School of Medicine and Dentistry, Dulwich Hospital, London, United Kingdom. w.githui@lshtm.ac.uk |
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| Abstract: | Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycobacterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with IS6110-specific primers yielded a 181-bp fragment only in DNA from M. tuberculosis complex isolates. |
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