|
|||||
|
|
| The effects of GM1 and bFGF synergistically inducing adultrat bone marrow stromal cells to form neural progenitor cells and their differentiation | |
| 引用本文： | 张卉,王纪佐,孙红宇,张建宁,杨树源. The effects of GM1 and bFGF synergistically inducing adultrat bone marrow stromal cells to form neural progenitor cells and their differentiation[J]. 中华创伤杂志(英文版), 2004, 7(1): 3-6 |
| 作者姓名： | 张卉 王纪佐 孙红宇 张建宁 杨树源 |
| 作者单位： | [1]TianjinHuanhuHospital,Tianjin300060,China [2]GeneralHospital,TianjinMedicalUniversity,Tianjin,China [3]SecondTeachingHospital,TianjinMedicalUniversity,Tianjin,China |
| 摘 要： | Objective: To investigate the effects of GM1 on inducing adult rat bone marrow stromal cells (MSCs) to form neural progenitor cells and their differentiation. Methods: Purified MSCs were induced by different components of basic fibroblast growth factor (bFGF) alone, GM1 alone or combination of bFGF with GM1. After 3 days‘ incubation, fibronectin and collagen I were detected with immunocytochemistry, and nestin was detected with immunofluorescence. Neuron-specific enolase ( NSE ), glial fibrillary acidic protein ( GFAP ) and galactose cerebroside ( GalC ) were detected with immunocytochemistry after 7 days‘ incubation. Results: After induction with bFGF alone or combination of bFGF and GM1, some MSCs exhibited the pbenotypes of neural progenitor cells, and then neurons and astrocytes. In these two groups, the positive cells for fibronectin and collagen I decreased markedly after 3 days‘ induction. At the same time, the positive cells for nestin increased markedly. After 7 days‘ induction, NSE and GFAP-positive cells increased significantly. Furthermore, the addition of bFGF and GM1 caused the maximal variation. However, addition of GM1 alone had no inductive effects. Conclusions: Combination of bFGF with GM1 may synergistically promote the transformation of MSCs and differentiation into neurons and astrocyte-like cells. The results suggest a promising route for the application of MSCs. |
| 关 键 词： | 基质纤维原细胞生长因子 骨髓基质细胞 神经祖细胞 细胞转换 GM1 细胞因子 作用机制 成年大鼠 |
The effects of GM1 and Bfgf synergistically inducing adult rat bone marrow stromal cells to form neural progenitor cells and their differentiation |
|
| ZHANG Hui ,WANG Ji zuo,SUN Hong yu,ZHANG Jian ning and YANG Shu yuan Tianjin Huanhu Hospital,Tianjin ,China. The effects of GM1 and Bfgf synergistically inducing adult rat bone marrow stromal cells to form neural progenitor cells and their differentiation[J]. Chinese journal of traumatology, 2004, 7(1): 3-6 | |
| Authors: | ZHANG Hui WANG Ji zuo SUN Hong yu ZHANG Jian ning YANG Shu yuan Tianjin Huanhu Hospital Tianjin China |
| Affiliation: | 1. Tianjin Huanhu Hospital, Tianjin 300060, China 2. Second Teaching Hospital, Tianjin Medical University, Tianjin, China 3. General Hospital, Tianjin Medical University, Tianjin, China |
| Abstract: | Objective: To investigate the effects of GM1 on inducing adult rat bone marrow stromal cells(MSCs) to form neural progenitor cells and their differentiation. Methods: Purified MSCs were induced by different components of basic fibroblast growth factor (bFGF) alone, GM1 alone or combination of bFGF with GM1. After 3 days' incubation, fibronectin and collagen I were detected with immunocytochemistry, and nestin was detected with immunofluorescence. Neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP) and galactose cerebroside (GalC) were detected with immunocytochemistry after 7 days' incubation. Results: After induction with bFGF alone or combination of bFGF and GM1, some MSCs exhibited the phenotypes of neural progenitor cells, and then neurons and astrocytes. In these two groups, the positive cells for fibronectin and collagen I decreased markedly after 3 days' induction. At the same time, the positive cells for nestin increased markedly. After 7 days' induction, NSE and GFAP positive cells increased significantly. Furthermore, the addition of bFGF and GM1 caused the maximal variation. However, addition of GM1 alone had no inductive effects. Conclusions: Combination of bFGF with GM1 may synergistically promote the transformation of MSCs and differentiation into neurons and astrocyte like cells. The results suggest a promising route for the application of MSCs. |
| Keywords: | Bone marrow Stem cells Transformations Differentiation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |