电针刺激"曲池"和"足三里"对大鼠脑梗死影响的形态学观察

目的观察电针刺激对脑缺血大鼠脑梗死面积和梗死边缘区神经细胞形态学的影响，为探讨针刺治病疗效和机理提供实验依据。方法线栓法建立大鼠脑梗死模型，用MRI、光学和透射电子显微镜观察电针后，模型大鼠脑梗死面积及梗死边缘区神经细胞及超微结构的变化。结果MRI显示电针组大鼠的梗死区面积比对照组的明显缩小（P〈0．01）；光学显微镜下，电针1d组梗死边缘区神经细胞形态的缺血性损伤与对照组ld相比无显著性差异（P〉0．05），电针组7d与对照组7d相比，细胞损伤程度和受损细胞数量，均有显著性差异（P〈0．01）；电子显微镜下，电针组缺血边缘区的神经细胞超微结构，如腺粒体的肿胀、嵴的破坏及核蛋白体变性程度均比对照组轻。结论电针穴位刺激能减小脑梗死面积并对梗死边缘区神经细胞提供一定的保护作用。

MORPHOLOGICAL OBSERVATION OF EFFECTS OF ELECTROACUPUNCTURE ON BRAIN INFARCT RAT

Objective To provide experiment basis for acupuncture therapy by observation of effects of electroacupuncture on infarct area and neuron morphology of brain penumbrazone in ischemia rat. Methods The brain infarct model of rat was established by thread embolism.Mangnetic resonance image,light and electron microscopy were used to observe the change of cerebral infarction area and neuron ultrastructure. Results 1.The infract area was decereased in acupuncture group comparing with control group by MRI study. 2.Results from light microscopy observation showed there was no difference between the damage neurons of penumbrazone in the acupuncture and control groups(P>0.05) 1 day after acupuncture.There was a significant difference in the damage neurons of penumbrazone between the acupuncture and control groups(P<0.01) 7 days after acupuncture. 3.Results from electron microscopy study showed the ultrastrustructure of neuron of penumbrazone,for example mitochondrion were less edema in the acupuncture group than the control group,so was the cristae of mitochondria and ribosome.Conclusion Electro-acupuncture acupoint can reduce cerebral infarct area and protect neurons of penumbrazone.