胶质母细胞瘤3号染色体杂合性丢失的研究

目的　寻找胶质母细胞瘤 (GBM) 3号染色体上可能存在肿瘤抑制基因的杂合性丢失 (LOH)区域 ,为发现和定位肿瘤抑制基因 (TSG)提供线索和依据。方法　采用荧光标记的引物和 377型DNA序列自动分析仪 ,分析了 2 0例GBM 3号染色体上 2 3个微卫星多态性标记的LOH。结果　在 50 % (1 0 / 2 0例 )GBM的 3号染色体上观察到LOH ,在 2 5 .6 % (88/ 344)可提供信息位点存在LOH。其中 3q的LOH率明显高于 3p ,3q和 3p的LOH率分别为 50 % (1 0 / 2 0 )、35 % (7/ 2 0 )。在 3q上的下列位点检测到较高的LOH率 :3q2 2 2 3上的微卫星位点D3s1 569(35 .3 % )、3q2 4 2 7上的D3s1 61 4 (42 .9% ) D3s1 565(35 .3 % ) ;在3p1 4 .1 1 4 .3上D3s1 2 89的LOH率也较高 (33 .3 % )。 结论　 3号染色体可能在GBM的分子发病机制中发挥着重要作用 ,3p1 4 .1 1 4 .3上的D3s1 2 89和 3q2 2 2 3上的D3s1 569位点、3q2 4 2 7上的D3s1 61 4 D3s1 565位点间区域可能存在与GBM相关的肿瘤抑制基因

A preliminary study of loss 9f heterozygosity on chromosome 3 in glioblastoma

Objective To detect loss of heterozygosity (LOH) in order to locate the deletion areas probably harboring tumor suppressor genes(TSGs) in glioblastoma (GBM). Methods PCR based microsatellite polymorphism analyses were performed to detect LOH on chromosome 3, fluorescence labeled primers and Perkin Elmer 377 DNA Sequencer were applied. Results 50% informative cases of GBM displayed LOH on chromosome 3. 25.6% of informative loci showed LOH in our series, in which the higher frequent LOH were observed in the chromosomal region from loci D3s1614(42.9%) to D3s1565 on 3q24 27 and at loci D3s1569(35.3%) on 3q22 23 and D3s1289 (33.3%) on 3p14.1 14.3. Conclusion Loss of genetic material on chromosome 3 may play an important role on the initiation and progression of GBM. The chromosomal regions from loci D3s1614 to D3s1565 on 3q24 27 and at loci D3s1569 on 3q22 23 and D3s14.3 may harbor novel tumor suppressor genes associated with GBM.