Carboxymethyl poly(L-histidine) as a new pH-sensitive polypeptide to enhance polyplex gene delivery |
| |
| Authors: | Asayama Shoichiro Sudo Miyuki Nagaoka Shoji Kawakami Hiroyoshi |
| |
| Institution: | Department of Applied Chemistry, Tokyo Metropolitan University, Hacioji, Tokyo, Japan. asayama-shoichiro@c.metro-u.ac.jp |
| |
| Abstract: | Carboxymethyl poly( l-histidine) (CM-PLH) as a new pH-sensitive polypeptide has enhanced polyplex gene delivery. Agarose gel retardation assay and zeta potential measurement proved that the anionic CM-PLH at physiological pH coated the PEI/DNA binary complexes. The resulting CM-PLH/PEI/DNA ternary complexes showed the gene expression value 300 times higher than that of the PEI/DNA binary complexes. These results suggest that the synergistic effect of the pH-sensitive imidazole groups at endosomal pH and the anionic carboxymethyl groups at physiological pH in the CM-PLH enhanced polyplex gene delivery. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
