| 七叶皂苷钠对小鼠肝癌H22移植瘤的抗肿瘤作用机制 |
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| 引用本文： | 聂昕,郭维,徐波,崔景荣. 七叶皂苷钠对小鼠肝癌H22移植瘤的抗肿瘤作用机制[J]. 中国新药杂志, 2011, 0(8) |
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| 作者姓名： | 聂昕 郭维 徐波 崔景荣 |
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| 作者单位： | 北京大学天然药物及仿生药物国家重点实验室; |
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| 基金项目： | 国家“重大新药创制”科技重大专项(2009ZX09301-010) |
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| 摘 要： | 目的:通过体内模型研探讨七叶皂苷钠对H22小鼠肝癌移植瘤的抗肿瘤作用机制。方法:采用ICR小鼠H22荷瘤肝癌模型观察七叶皂苷钠抗肿瘤作用,采用Western Blot分析肿瘤组织中相关蛋白的表达变化,通过免疫组化分析肿瘤组织中CD31变化,评价微血管密度。结果:1.4和2.8 mg.kg-1七叶皂苷钠对H22的抑瘤率分别为19.2%,40.7%。免疫组化结果显示,七叶皂苷钠能够显著降低肿瘤内部微血管密度,低高剂量组可分别达到44.1%和48.5%。Western Blot证实,七叶皂苷钠能不同程度下调cyclinD1、cdk2、cyclinE、VEGF、p-Akt等蛋白的表达,抑制p65的核转位。结论:七叶皂苷钠有一定的抗肿瘤作用,其机制可能与阻滞细胞周期,抑制血管新生以及干扰信号转导有关。
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| 关 键 词： | 七叶皂苷钠 细胞周期相关蛋白 微血管密度 NFκ-B 血管内皮生长因子 |
Antitumor effect of sodium aescinate in mice with H22 hepatoma and the mechanism |
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| NIE Xin,GUO Wei,XU Bo,CUI Jing-rong. Antitumor effect of sodium aescinate in mice with H22 hepatoma and the mechanism[J]. Chinese Journal of New Drugs, 2011, 0(8) |
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| Authors: | NIE Xin GUO Wei XU Bo CUI Jing-rong |
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| Affiliation: | NIE Xin,GUO Wei,XU Bo,CUI Jing-rong(National Research Laboratory of Natural and Biomimetic Drugs,Peking University,Beijing 100083,China) |
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| Abstract: | Objective:To investigate the antitumor effect of sodium aescinate in vivo and the mechanisms.Methods: A mouse model of H22 hepatoma was employed to investigate the antitumor effect of sodium aescinate.Samples from tumor tissue were analyzed for the tumor growth associated proteins through Western Blot.Immunohistochemistry was performed to evaluate the microvessel density through CD31 labeling.Results: Sodium aescinate inhibited tumor growth and reduced tumor weight by 19.2% and 40.7% at doses of 1.4 and 2.8... |
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