| 微柱离心-紫外分光光度法测定超氧化物歧化酶模拟物脂质体的包封率 |
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| 引用本文： | 陈建霞,徐缓,于涛,王澈,由忠路. 微柱离心-紫外分光光度法测定超氧化物歧化酶模拟物脂质体的包封率[J]. 中国新药杂志, 2011, 0(10) |
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| 作者姓名： | 陈建霞 徐缓 于涛 王澈 由忠路 |
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| 作者单位： | 辽宁师范大学化学化工学院; |
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| 摘 要： | 目的:建立超氧化物歧化酶(superoxide dismutase,SOD)模拟物脂质体包封率的测定方法。方法:利用紫外分光光度法测定SOD模拟物的含量,检测波长为371 nm。以薄膜分散法制备SOD模拟物脂质体,采用葡聚糖凝胶-微柱离心-紫外分光光度法测定其包封率。结果:SOD模拟物在60.0～240.0μg.mL-1范围内线性关系良好(r=0.999 3),微柱离心法能有效地将脂质体与游离药物分离,加样回收率测定结果为(99.18±1.05)%,利用该方法测定的SOD模拟物脂质体包封率为(91±1.63)%。结论:紫外分光光度法可以作为SOD模拟物的含量测定方法,微柱离心法操作简单,重现性好,适用于SOD模拟物脂质体包封率的测定。
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| 关 键 词： | SOD模拟物 脂质体 薄膜分散法 包封率 微柱离心法 |
Determination of entrapment efficiency of superoxide dismutase mimic liposomes by mini-column centrifugation-UV spectrophotometry |
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| CHEN Jian-xia,XU Huan,YU Tao,WANG Che,YOU Zhong-lu. Determination of entrapment efficiency of superoxide dismutase mimic liposomes by mini-column centrifugation-UV spectrophotometry[J]. Chinese Journal of New Drugs, 2011, 0(10) |
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| Authors: | CHEN Jian-xia XU Huan YU Tao WANG Che YOU Zhong-lu |
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| Affiliation: | CHEN Jian-xia,XU Huan,YU Tao,WANG Che,YOU Zhong-lu(College of Chemistry and Chemical Engineering,Liaoning Normal University,Dalian 116029,China) |
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| Abstract: | Objective: To establish a method for determination of entrapment efficiency of superoxide dismutase(SOD) mimic liposomes.Methods: The concentration of SOD mimics was determined by UV spectrophotometry,and the UV detective wavelength was set at 371 nm.SOD mimic liposomes were prepared by the film-dispersion method,and the entrapment efficiency of the liposomes was determined by Sephadex G-50 mini-column centrifugation-UV spectrophotometry.Results: A calibrated linear curve of SOD mimic concentrations was wit... |
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| Keywords: | superoxide dismutase mimics liposomes film-dispersion method entrapment efficiency mini-column centrifugation method |
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