| 人参次苷H滴丸定性与定量方法研究 |
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| 引用本文： | 张兰兰,高文远,宋兆辉,蔡楠,黄芝娟.人参次苷H滴丸定性与定量方法研究[J].中国新药杂志,2011(9). |
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| 作者姓名： | 张兰兰 高文远 宋兆辉 蔡楠 黄芝娟 |
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| 作者单位： | 天津大学药物科学与技术学院;天津天士力集团现代中药研究所; |
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| 基金项目： | 国家“重大新药创制”科技重大专项(2008ZX09401-006) |
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| 摘 要： | 目的:建立人参次苷H滴丸质量控制方法。方法:采用薄层色谱法同时鉴别人参皂苷Rh1和Rh2;采用香草醛冰醋酸-高氯酸比色法测定总皂苷含量;采用高效液相色谱法测定人参皂苷Rh1和Rh2含量。结果:人参皂苷Rh1和Rh2样品分离较好,薄层色谱斑点清晰;总皂苷含量测定的平均回收率为98.81%,线性范围为0.056~0.448 mg.mL-1;人参皂苷Rh1线性回归方程y=6 462.7x+2.073 5,当进样量在0.208~4.16μg范围内,与其峰面积呈线性,r=0.9997;人参皂苷Rh2线性回归方程y=9867.2x+0.1204,当进样量在1.148~22.96μg范围内,与其峰面积呈线性,r=0.999 9。结论:建立的人参次苷H滴丸质控方法准确、专属性及重现性好且快速简便,可用于该制剂的质量控制。
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| 关 键 词： | 人参次苷H滴丸 人参皂苷Rh1 人参皂苷Rh2 |
Qualitative and quantitative study for secondary ginsenoside H dripping pills |
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| ZHANG Lan-lan,GAO Wen-yuan,SONG Zhao-hui,CAI Nan,HUANG Zhi-juan.Qualitative and quantitative study for secondary ginsenoside H dripping pills[J].Chinese Journal of New Drugs,2011(9). |
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| Authors: | ZHANG Lan-lan GAO Wen-yuan SONG Zhao-hui CAI Nan HUANG Zhi-juan |
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| Institution: | ZHANG Lan-lan1,2,GAO Wen-yuan1,SONG Zhao-hui2,CAI Nan2,HUANG Zhi-juan2(1 College of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China,2 Institute of Modern TCM,Tianjin Tasly Group,Tianjin 300410,China) |
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| Abstract: | Objective:To study the quality control methods for secondary ginsenoside H dripping pills.Methods:Ginsenosides,Rh1 and Rh2,were simultaneously identified by thin layer chromatography(TLC).The contents of Rh1 and Rh2 were determined by high performance liquid chromatography(HPLC).Results:Rh1 and Rh2 in samples were separated.The TLC spots were clear.The equation of linear regression was as follows:y=6 462.7x+2.073 5(Rh1) and y=9 867.2x+0.120 4(Rh2).Rh1 and Rh2 showed a good linear relationship in the range o... |
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| Keywords: | secondary ginsenoside H dripping pills ginsenoside Rh1 ginsenoside Rh2 |
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