| 血管活性肠肽化学及生物学稳定性研究 |
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| 引用本文： | 崔旭,韩璇,王志敏,曹德英,郑爱萍. 血管活性肠肽化学及生物学稳定性研究[J]. 中国新药杂志, 2011, 0(19) |
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| 作者姓名： | 崔旭 韩璇 王志敏 曹德英 郑爱萍 |
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| 作者单位： | 河北医科大学药学院;河北大学医学部基础医学院;军事医学科学院毒物药物研究所; |
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| 基金项目： | 河北省自然科学基金(C2009001066) |
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| 摘 要： | 目的:考察血管活性肠肽(vasoactive intestinal peptide,VIP)的化学及生物学的稳定性,为VIP的制剂学研究提供依据。方法:考察VIP在不同pH值(2.0,4.0,7.0,9.0,11.0,13.0)、不同离子强度溶液、不同温度以及人工胃液和人工肠液中的稳定性,用HPLC法检测VIP含量变化。结果:VIP的稳定性具有pH依赖性,VIP在酸性及中性条件下稳定,pH≤7时几乎无降解,但VIP在碱性条件下不稳定,pH=13时30min已完全降解;离子强度对其稳定性无影响;VIP在冷冻条件下稳定性良好,在冷藏条件下低浓度存在降解;VIP在人工胃液和人工肠液中降解迅速,0 min即完全降解,无法检测到主药峰。结论:VIP化学及生物学的稳定性差,口服无效。
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| 关 键 词： | 血管活性肠肽 稳定性 人工胃液 人工肠液 |
Chemical and biological stability of vasoactive intestinal peptide |
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| CUI Xu,HAN Xuan,WANG Zhi-min,CAO De-ying,ZHENG Ai-ping. Chemical and biological stability of vasoactive intestinal peptide[J]. Chinese Journal of New Drugs, 2011, 0(19) |
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| Authors: | CUI Xu HAN Xuan WANG Zhi-min CAO De-ying ZHENG Ai-ping |
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| Affiliation: | CUI Xu1,HAN Xuan2,WANG Zhi-min1,CAO De-ying1,ZHENG Ai-ping3(1 College of Pharmaceutical Science,Hebei Medical University,Shijiazhuang 050017,China,2 Hebei University Health Science Center,Baoding 071000,3 Institution of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850,China) |
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| Abstract: | Objective: To investigate the chemical and biological stability of vasoactive intestinal peptide(VIP) for providing a theory basis for pharmaceutical development of VIP.Methods: The stability of VIP in different pH values,ionic strength,temperature,artificial gastric fluid and artificial intestinal fluid was investigated.The concentration of VIP was determined by HPLC method.Results: The stability of VIP was pH-dependent.VIP was stable in acid and neutral solutions;almost no degradation was found in solutio... |
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| Keywords: | vasoactive intestinal peptide stability artificial gastric fluid artificial intestinal fluid |
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