| 碳青霉烯类抗生素体外抗菌活性及其耐药机制研究 |
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| 引用本文： | 韩慧,胡辛欣,李聪然,杨信怡,娄人慧,张伟新,游雪甫.碳青霉烯类抗生素体外抗菌活性及其耐药机制研究[J].中国新药杂志,2011(12). |
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| 作者姓名： | 韩慧 胡辛欣 李聪然 杨信怡 娄人慧 张伟新 游雪甫 |
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| 作者单位： | 中国医学科学院&北京协和医学院医药生物技术研究所药理室; |
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| 基金项目： | 国家“重大新药创制”科技重大专项(2009ZX09303-005,2008ZX09305-001,2009ZX09301-003); 国家自然科学基金(30701062,30901876) |
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| 摘 要： | 目的:研究碳青霉烯类抗生素对临床分离菌的体外抗菌活性,分析其耐药机制。方法:采用平皿二倍稀释法测定4种碳青霉烯类抗生素对352株临床菌株的MIC值,应用PCR方法鉴定铜绿假单胞菌、鲍曼不动杆菌的耐药基因。结果:4种药物对除铜绿假单胞菌和鲍曼不动杆菌外的其他菌种非常敏感。从两种耐药菌中分别鉴定出VIM-2和OXA-23基因。结论:碳青霉烯类抗生素对大部分临床分离菌株的抗菌活性较好。OXA-23基因为鲍曼不动杆菌耐药的主要机制之一。
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| 关 键 词： | 碳青霉烯类抗生素 临床菌株 耐药 鲍曼不动杆菌 铜绿假单胞菌 |
In vitro activity of carbapenems and their resistance mechanism |
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| HAN Hui,HU Xin-xin,LI Cong-ran,YANG Xin-yi,LOU Ren-hui,ZHANG Wei-xin,YOU Xue-fu.In vitro activity of carbapenems and their resistance mechanism[J].Chinese Journal of New Drugs,2011(12). |
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| Authors: | HAN Hui HU Xin-xin LI Cong-ran YANG Xin-yi LOU Ren-hui ZHANG Wei-xin YOU Xue-fu |
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| Institution: | HAN Hui,HU Xin-xin,LI Cong-ran,YANG Xin-yi,LOU Ren-hui,ZHANG Wei-xin,YOU Xue-fu(Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100050,China) |
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| Abstract: | Objective: To determine the sensitivity and resistance in clinical strains to carbapenems,and investigate their carbapenemase genotypes.Methods: Totally 352 clinically isolated strains were collected from hospitals in Beijing,2007-2009.Their MIC to meropenem,imipenem/cilastatin,panipenem and faropenem were detected by the agar dilution method.The highly resistant strains,Pseudomonas aeruginosa and Acinetobacter baumannii,were chosen for PCR screening to find the carbapenemase genes.Results: Meropenem,imipen... |
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| Keywords: | carbapenems clinical isolated strains drug-resistance Acinetobacter baumannii Pseudomonas aeruginosa |
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