| 曲古菌素A诱导急性髓性白血病细胞表达共刺激分子CD80和CD86的研究 |
| |
| 引用本文： | 余美霞,郭伟,周浩,周咏明.曲古菌素A诱导急性髓性白血病细胞表达共刺激分子CD80和CD86的研究[J].中国新药杂志,2011(4). |
| |
| 作者姓名： | 余美霞 郭伟 周浩 周咏明 |
| |
| 作者单位： | 武汉科技大学附属天佑医院妇产科;九江学院附属医院妇产科;武汉市第一医院;华中科技大学同济医学院附属协和医院血液科;武汉科技大学附属天佑医院血液科; |
| |
| 基金项目： | 江西省自然科学基金(0640190); 湖北省教育厅资助基金(530026) |
| |
| 摘 要： | 目的:研究组蛋白去乙酰化酶抑制剂曲古菌素A(trichostatin A,TSA)诱导急性髓性白血病细胞表达共刺激分子CD80和CD86。方法:流式细胞仪检测TSA诱导HL-60细胞、急性髓性白血病患者单个核细胞表达共刺激分子CD80和CD86及细胞活力;RT-PCR法分析CD80和CD86 mRNA的表达情况;75Gy(gray,Gy)照射TSA诱导的HL-60细胞后,采用CCK-8法检测共刺激分子表达增高后,HL-60细胞对健康人单核细胞的增殖作用。结果:TSA使HL-60细胞CD86的表达上调,也可急性髓性白血病患者单核细胞CD86的表达上调,TSA诱导HL-60细胞表达共刺激分子CD86后,可促进健康人单核细胞的增殖。结论:TSA诱导HL-60细胞、急性髓性白血病患者单核细胞共刺激分子CD86表达增高,促进健康人单核细胞的增殖。
|
| 关 键 词： | 曲古菌素A 抗原 CD80 CD86 淋巴细胞反应 |
Trichostatin A induces expression of costimulatory molecules CD80 and CD86 in acute myelocytic leukemia cells |
| |
| YU Mei-xia,GUO Wei,ZHOU Hao,ZHOU Yong-ming.Trichostatin A induces expression of costimulatory molecules CD80 and CD86 in acute myelocytic leukemia cells[J].Chinese Journal of New Drugs,2011(4). |
| |
| Authors: | YU Mei-xia GUO Wei ZHOU Hao ZHOU Yong-ming |
| |
| Institution: | YU Mei-xia1,2,GUO Wei3,ZHOU Hao3,ZHOU Yong-ming4(1 Department of Gynecology and Obstetrics,Affiliated Tianyou Hospital,Whuhan University of Science and Technology,Wuhan 430064,China,2 Affiliated Hospital,Jiujiang Unversity,Jiujiang 332000,3 Department of Endocriology,Wuhan First Hospital,Wuhan 430022,4 Department of Hematology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,5 Department of Hematology,Whuhan Univer... |
| |
| Abstract: | Objective: To investigate trichostatin A(TSA)-induced expression of costimulatory molecules CD80 and CD86 in acute myelocytic leukemia(AML) cells.Methods: The expression of CD80 and CD86 was detected,and cell viability was examined by flow cytometry in HL-60 cells and in mononuclear cells of bone marrow in AML patients after TSA treatment.The expression of CD86 mRNA was analyzed by RT-PCR.After TSA-treated HL-60 cells were irradiated(75Gy),their effect on proliferation of peripheral blood mononuclear cells(... |
| |
| Keywords: | trichostatin A antigen CD80 CD86 allogeneic mixed lymphocyte reaction |
| 本文献已被 CNKI 等数据库收录！ |
|
