佳蓉片中6种乌头类生物碱的定量研究

目的分析佳蓉片中苯甲酰新乌头原碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、新乌头碱、乌头碱和次乌头碱6种乌头类生物碱的含量。方法采用HPLC法。色谱条件:Kromasil C₁₈色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-四氢呋喃(25∶15)以及0.1 mol·L^-1醋酸铵溶液(每1 000 mL加冰醋酸0.5 mL),梯度洗脱;流速为0.8 mL·min^-1;柱温为25℃;检测波长为235 nm。结果苯甲酰新乌头原碱、苯甲酰乌头原碱、苯甲酰次乌头原碱、新乌头碱、乌头碱和次乌头碱分别在4.32～432.40,1.29～128.83,1.08～108.46,1.77～177.30,0.78～78.40和1.19～119.04μg·mL^-1范围内与峰面积线性关系良好;平均回收率分别为96.46%,98.26%,98.03%,98.23%,98.86%和98.70%;RSD值分别为1.50%,1.20%,1.27%,1.06%,1.50%和1.19%;10批制剂中苯甲酰新乌头原碱、苯甲酰乌头原...

Quantitative study on 6 aconitoid alkaloids in Jiarong Tablets

Objective To analyze the contents of 6 kinds of aconitoid alkaloids including benzoylmesaconine,benzoylaconitine,benzoylhypacoitine,mesaconitine,aconitine and hypaconitine in Jiarong Tablets.Methods HPLC was adopted,and the chromatographic column was Kromasil C 18(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile-tetrahydrofuran(25∶15)and 0.1 mol·L^-1 ammonium acetate solution(0.5 mL glacial acetic acid per 1000 mL)in a gradient mode.The flow rate was 0.8 mL·min-1.The column temperature was 25℃ and the detection wavelength was 235 nm.Results The mass of benzoylmesaconine,benzoylaconitine,benzoylhypacoitine,mesaconitine,aconitine and hypaconitine showed good linear relationships with peak area in the range of 4.32-432.40,1.29-128.83,1.08-108.46,1.77-177.30,0.78-78.40 and 1.19-119.04μg·mL^-1,respectively.Their average recoveries were 96.46%,98.26%,98.03%,98.23%,98.86%and 98.70%,respectively with RSD were 1.50%,1.20%,1.27%,1.06%,1.50%and 1.19%,respectively.The content range of benzoylmesaconine,benzoylaconitine,benzoylhypacoitine,mesaconitine,aconitine and hypaconitine in 10 batches of preparation were 100.32-119.18,9.35-16.02,7.99-10.87,1.54-1.85,0.87-0.99 and 2.21-3.27μg·g^-1,respectively.Conclusion This method is simple,accurate,sensitive and reproducible.And it can be used as a quantitative method for aconitoid alkaloids in Jiarong Tablets.