机体自发清除丙型肝炎病毒和主要组织相容性复合体区域单核苷酸多态性关联的初步研究简

目的了解中国丙型肝炎病毒（hepatitisCvirus，HCV）感染人群机体自发清除HCV和主要组织相容性复合体（major histocompatibility complex，MHC）区域单核苷酸多态性（single—nucleotide polymorphism，SNP）的关联性。方法选择96名抗-HCV阳性并成功随访1年的献血者为研究对象，分为自发清除组（HCVRNA阴性）和慢性感染组（HCVRNA阳性），采用Illumina MHC Mapping Panel试剂盒对两组人群进行MHC区域的SNPs检测，利用SPSS16．0分析软件、Matlab软件编程，采用t检验、x2检验及分层分析等方法对各SNP位点及性别、年龄因素与HCV清除与否进行关联性分析。结果获得HCV自发清除组和慢性感染组样本分别为19份和77份。两组间年龄差异无统计学意义（t=0．610，P=0．543），HCV自发清除组中女性感染者的比例明显高于男性（x2=17．668，P=2．63×10^-5）。检测的1293个SNPs中，rs2246626（x2=10．777，P=0．001）、rs12386522（x2=7．725，P=0．005）、rs2534679（x2=7．522，P=0．006）和rs1264457（x2=6．821，P=0．009）与HCV的自发清除显著相关。结论MHC区域共发现4个与HCV自发清除存在显著性关联的SNPs，女性发生HCV自发清除比例高于男性。该结果将为进一步研究机体自发清除HCV和MHC基因的关联奠定基础。

Spontaneous resolution of hepatitis C and its association with SNPs of the MHC gene in Guangzhou,China

Objective To study the association between the spontaneous resolution of hepatitis C and the singlenucleotide polymorphisms （SNPs） in the major histocompatibility complex （MHC） gene in hepatitis C virus （HCV） -infected individuals in Guangzhou, China. Methods A total of 96 voluntary blood donors with posi- tive anti HCV were recruited and followed-up for one year. Donors were divided into spontaneously resolved HCV persistence groups. SNPs of MHC gene were detected using Illumina MHC Mapping Panel Test and data analysis was done with Matlab programming, t-test, ehi-square test and stratification analysis. Results Among the 96 subjects, 19 were spontaneously resolved and 77 turned to HCV persistent. There was no significant difference in ages between these two groups （t=0. 610, P=0. 543） but the proportion of females was significantly higher in the spontaneously resolved group than that in the HCV persistent group （x2 = 17. 668, P= 2.63 × 10^-5）. Of all 1 293 SNPs detected, rs2246626 （x2= 10.777, P=0.001）, rs12386522 （x2=7.725, P=0.005）, rs2534679 （x2 =7. 522, P=0. 006） and rs1264457 （x2=6. 821, P=0. 009） were found to be associated with HCV spontaneous resolution. Conclusions Four MHC SNPs are associated with the HCV spontaneous resolution in Guangzhou, China. Women seem more likely to have the HCV infection resolved spontaneously.