内皮素A受体拮抗剂BQ123对前列腺癌PC3细胞增殖与凋亡的影响

目的:观察内皮素A受体拮抗剂BQ123对人前列腺癌(PCA)PC3细胞株增殖与凋亡的影响,探讨内皮素A受体拮抗剂对PCA细胞的体外抗肿瘤效应。方法:采用四甲基偶氮唑蓝(MTT)比色法、划痕损伤实验、细胞迁移实验观察BQ123对人PCA PC3细胞株增殖的抑制效应,利用Annexin V-FITC/PI染色和流式细胞术检测BQ123诱导肿瘤细胞凋亡的作用及其与细胞周期的关系。结果:BQ123对PCA PC3细胞株有抑制作用,并表现出良好的时效关系。细胞凋亡和细胞周期实验表明,BQ123可诱导PC3细胞株的凋亡,其诱导细胞凋亡的作用与细胞周期无关。结论:BQ123对PCA PC3细胞株增殖的抑制效应具有时间依赖性,并可诱导细胞凋亡,可为PCA的治疗提供一种新的思路。

Endothelin A receptor antagonist BQ123 inhibits the proliferation and induces the apoptosis of human prostate cancer cell line PC3

Objective:To investigate the anti-tumor effect of the endothelin A receptor antagonist BQ123 on the human prostate cancer cell line PC3 in vitro by observing its impact on the proliferation and apoptosis of human prostate cancer cells.Methods:The inhibiting effect of BQ123 on the proliferation of PC3 cells was observed by MTT assay,erasion trace test and Traswell chamber chemotaxis assay,and its induction of their apoptosis determined by Annexin V-FITC/PI staining and flow cytometry.Results:BQ123 inhibited the migration and invasion abilities of PC3 cells in a dose-dependent manner and induced their apoptosis as well.Conclusion:BQ123 inhibits the proliferation of PC3 cells in a dose-dependent manner and induces their apoptosis in vitro,which has offered a new idea for the treatment of prostate cancer.