GABA对黄体细胞孕酮分泌及凋亡的影响

目的：探讨ＧＡＢＡ对大鼠黄体细胞孕酮生成的影响及其机制，孕酮生成与细胞凋亡的关系。方法：未成年Ｗｉｓｔａｒ雌性大鼠，经ＰＭＳＧ－ｈＣＧ顺序处理后，取出双侧卵巢制成黄体细胞悬浮液，进行细胞培养，放射免疫分析方法测定激素含量，３’－末端原位标记法检测离体培养黄体细胞自发凋亡，结果：⑴ＧＡＢＡ抑制黄体细胞基础孕酮的生成及ｈＣＧ刺激下的孕酮生成，⑵Ｆｏｒｓｋｌｉｎ促进黄体细胞孕酮的生成，ＧＡＢＡ抑制Ｆｏｒ

Effect of GABA on progesterone secretion and apoptosis by cultured rat luteal cells

The present experiment was carried out to study the effect of GABA on progesterone secretion and apoptosis by incubated luteal cells of rat in vitro.Methods: I m m a t u r e f e m a l e r a t s w e r e t r e a t e d w i t h P M S G a n d H C G i n d u c e h y p e r o v u l a t i o n f o r a b t a i n i ng l u t e a l c e l l s .T h e p r o d u c t i o n o f p r o g e s t e r o n e w e r e e l e t e m i n e d b y R I A T U N E L t e c h n i c w as u s e d t o d e t e c t t h e a p o t o t i c c e l l s.Results:1.GABA inhibited progesterone production and hCG-induced progesterone production by cultured rat luteal cells.2.GABA inhibited forskolin- induced progesterone production by cultured rat luteal cells.3.GABA enhanced apoptosis of l u t e a l c e l l s.Conclusion:GABA inhibited progesterone production by cultured rat luteal c e l l .T h e e f f e c t o f G A B A m i g h t b e m e d i a t e d b y G A B A r e c e p t o r a n d a d e n y l a t e c y c l a s e -c A MP messenger system.