肉苁蓉多糖的巨噬细胞活化作用

目的观察肉苁蓉多糖(CDPS)对巨噬细胞的活化作用。方法采用中性红法测定吞噬活性;Griess试剂法测定NO释放量;L929细胞法及小鼠胸腺细胞法测定TNF-α及IL-1释放。结果CDPS在6.25～50mg.L-1剂量范围内可剂量依赖性地增强BALB/c小鼠腹腔巨噬细胞吞噬中性红能力和促进NO释放;在2.8～100mg.L-1剂量范围内可使正常和免疫抑制的RAW264.7细胞NO释放明显增加;在0.56～7.2mg.L-1或3.6～10mg.L-1范围促进RAW264.7细胞TNF-α或IL-1产生,均具有良好的量效关系。结论CDPS可明显提高巨噬细胞吞噬及分泌功能,活化巨噬细胞。肉苁蓉免疫增强作用可能与此有关。

The effect of Cistanche deserticola polysaccharides(CDPS) on marcrophages activation

Aim To investigate the activation effect of Cistanche deserticola polysaccharides(CDPS) on marcrophages.Methods The phagocytic ability of peritoneal macrophage(MΦ) was evaluated with neutral red dye phagocytosis assay;nitric oxide(NO) production was examined by Griess reaction;the activity of tumor necrosis factor-α(TNF-α) and interleukin-1(IL-1) secreted were measured by L929 cells bioassay and proliferation of mouse thymocyte.Results CDPS at the concentration range of 6.25～50 mg·L-1 enhanced the phagocytic ability and NO release of peritoneal macrophage in a dose-dependent manner.CDPS at the dose of 2.8～100 mg·L-1 significantly increased NO secretion of normal or immunesuppressed RAW264.7 cells.TNF-α production by RAW264.7 cells was promoted after incubation of RAW264.7 cells with CDPS at the concentration of 0.56～7.2 mg·L-1.IL-1 release of RAW264.7 cells was obviously elevated by CDPS at the dose of 3.6～10 mg·L-1,and they both showed a good dose-effect relationship.Conclusions CDPS can markedly promote the phagocytic and secretory functions of MΦ,and activate MΦ,which suggests that the immunomodulatory effect of CDPS may be related to it.