荧光原位杂交法预测肿瘤放射敏感性的初步研究

目的 研究应用荧光原位杂交（FISH）方法预测人肿瘤细胞放射敏感性及其应用的可行性。方法 用3种放射敏感性不同的人肿瘤细胞株鼻咽鳞癌（CNE）、肺腺癌（SPC）和乳腺癌（MCF-7）]，常规克隆形成方法测定不同剂量照射后的存活分数和照射后24h经秋水仙素阻断细胞分裂周期，低渗、固定、常规染色体制片后，采用8号染色体涂染探针和FISH方法测定肿瘤细胞8号染色体诱导的畸变量。结果 2、4、6Gy照射后24h、CNE、SPC和MCF-7细胞诱导生成的残存染色体畸变能够反映细胞 的放射敏感性，所有细胞株诱导染色体畸变与细胞存活分数分别存在良好相关性（r=0.98），3种细胞株SF2和相应残存染色体畸变也存在良好相关性（r=0.96）。结论 采用FISH方法计数照射诱导的残存染色体畸变，可以预测肿瘤细胞的放射敏感性差异并具有重要的临床意义。

Preliminary study on prediction of radiosensitivity of human tumor cells with fl uorescence in situ hybridization

Objective　 To investigate whether chromosome induced-aberration s scored with fluorescence in situ hybridization (FISH) predict the radiosensiti vity of human tumor cell lines. 　Methods　 Three human cell lines with different radiosensitivities(nasopharyngeal cell line CNE, lung adenocarcinoma cell line SPC, and breast adenocarcinoma cell line MCF-7) were used for clonogenic assay . Cells were irradiated with a dose of 1～8?Gy. Radiation-induced aberration in a single chromosome (number 8) was measured at 24?h after irradiation of 2, 4 an d 6?Gy. 　Results　 Radiation-induced aberration in chromosome 8 reflected the rad i osensitivity in CNE, SPC and MCF-7 cells 24 h after irradiation. It was associa t ed with survival fraction at 2?Gy (r=0.96). 　Conclusion　These results suggest th at the radiation induced-chromosome aberrations in interphase measured by FISH be a considerable promise for prediction of radiosensitivity in tumor cells.