辣根过氧化物酶/吲哚乙酸系统作为基因导向性酶前体药物疗法的研究

目的:研究辣根过氧化物酶(HRP)/吲哚乙酸(IAA)系统作为基因导向性酶前体药物疗法(GDEPT)体外作用于人喉鳞癌Hep-2细胞株所产生的细胞毒性作用。方法:构建pcDNA3.1-HRP,将其瞬时转染Hep-2细胞后,Western blotting检测HRP蛋白的表达,MTT法检测加药IAA后对细胞增殖的影响。结果:pcDNA3.1-HRP构建成功,瞬时转染Hep-2细胞后Western blotting能检测到HRP蛋白的表达,MTT法显示HRP/IAA系统能较明显抑制细胞增殖。结论:HRP/IAA系统具备一定的作为基因导向性酶前体药物疗法治疗肿瘤的潜力。

Study of Horseradish Peroxidase/Indole-3-acetic Acid System in Gene-directed Enzyme Prodrug Therapy

Objective: To study the cytotoxicity on human laryngeal squamous carcinoma cell line Hep-2 using horseradish peroxidase(HRP)/ indole-3-acetic acid(IAA)system as gene-directed enzyme prodrug therapy(GDEPT).Methods: The vector,pcDNA3.1-HRP,was constructed and transiently transfected into Hep-2 cells.The HRP protein in Hep-2 cells was measured by Western blotting and the cell proliferation after IAA administration was measured by MTT assay.(Results:) The vector,pcDNA3.1-HRP was constructed successfully.The HRP expression was confirmed by Western blotting after cell transfection.The results of MTT assay indicated that HRP/IAA system could significantly inhibit cell proliferation.Conclusion: The results showed that the HRP/IAA system had the potential for the further use in cancer GDEPT.