瘤体内直接注射白介素2质粒复合物治疗小鼠肝癌

目的研究瘤体内直接注射白介素2质粒/阳离子脂质体复合物治疗小鼠肝癌的效果.方法将小鼠白介素2表达质粒(VR1110)与阳离子脂质体(Transfectam)按适当比例混合而形成复合物(VR1110/Transfectam).通过瘤体内直接注射此复合物治疗小鼠肝癌模型,并和卡介苗(BCG)的治疗相比较.结果瘤体内注射VR1110/Transfectam后,可在肿瘤组织中检测到小鼠IL-2 mRNA的表达.VR1110/Transfectam治疗组以及VR111/Transfectam/BCG治疗组肿瘤生长较其它组明显减慢,均显著提高小鼠的生存率(R＜0.05),但二者相差不明显.结论瘤体内直接注射VR1110/Transfectam复合物对小鼠肝癌的治疗作用明显,效果优于卡介苗,且方法简单,适合临床应用.

Treatment of Established Hepatic Tumor in Mice by Intratumoral Injection of Interleukin-2 Plasmid DNA/Lipid Complexes

Objective:To study the effect of interleukin-2plasmid DNA/lipid complexes for established hepatic tumor in mice by intratumoral injection.Methods:DNA-lipid complexes were formed by mixing VR1110and Transfectam at appropriate proportion.It was used in the treatment of established hepatic tumor in mice by intratumoral injection and it was compared with Bacillus Calmette-Guerin(BCG).Results:The intratumoral injection of VR1110/Transfectam complexes resulted in the expression of mRNA,significant reduction of tumor size and prolonged survival of mice(P<0.05).The same result was found after intratumoral injection of VR1110/Transfectam complexes in combination with BCG,but there was no significance between these two groups.Conclusion:The intratumoral injection of VR1110/Transfectam complexes can lead to a significant antitumor response in hepatoma bearing mice and its effect is better than the BCG's.This method is simple and suitable for clinical tumor therapy.