Type III hyperlipoproteinemia: a comparative study of current diagnostic techniques. |
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Authors: | J J Albers G R Warnick W R Hazzard |
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Affiliation: | Laboratoire de Biochimie C, Cliniques St. Charles, 34000 MontpellierFrance |
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Abstract: | Research into the prevalence, genetic transmission and pathophysiology of Type III hyperlipoproteinemia has suffered from the lack of a practical specific diagnostic procedure. In this study, very low density lipoprotein (VLDL) compositional criteria were established in a population lacking the beta-migrating VLDL characteristic of this disorder. Diagnosis by these criteria was compared to diagnosis using current criteria for the Type III lipoprotein pattern. In addition two techniques for detecting Type III without preliminary VLDL isolation by ultracentrifugation were evaluated. Plasma triglyceride (TG) concentration dependent cutlines for the compositional criteria reduced false positives at low TG levels and false negatives at high TG levels. Furthermore, an agarose electrophoresis heparin-manganese precipitation technique was effective for screening for a possible Type III pattern in plasma whereas the combination agarose-polyacrylamide gel electrophoresis system was not effective. |
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Keywords: | Address reprint requests to: Dr. John Albers Northwest Lipid Research Clinic Harborview Medical. Center 325 Ninth Avenue Seattle Washington 98104 U.S.A.. |
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