(-),(+)-7-羟基-黄皮酰胺对大鼠海马齿状回突触传递功能的影响(-),(+)-7-羟基-黄皮酰胺对大鼠海马齿状回突触传递功能的影响

目的观察(-),(+)-7-羟基-黄皮酰胺(7-hydoxy-clausenamide,7-OH-Clau)对大鼠海马齿状回基础突触传递功能的影响。方法细胞外记录低频刺激(1/30 Hz,1.0 mA)引起的海马齿状回群峰电位(population spike, PS)。结果侧脑室注入脑终浓度为2×10^-6 mol·L^-1 (-)-7-OH-Clau后15,30,60 min,PS幅值比给药前均增加了30%以上,比空白对照组增加了27%～41%;而同剂量的(+)-7-OH-Clau在注射后的3个时间点上,PS幅值比给药前下降了18%～25%,比空白对照组下降了11%～20%。结论(-)-7-OH-Clau能增强大鼠海马齿状回的基础突触传递活动,而(+)-7-OH-Clau对这种活动显示出抑制作用。

Effects of (-), (+)-7-hydroxy-clausenamide on synaptic transmission in rat dentate gyrus in vivo

AIM: To investigate effects of (-), (+)-7-hydroxy-clausenamide (7-OH-Clau) on basal synaptic transmission in the dentate gyrus of anesthetized rats. METHODS: Extracellular recording of the population spike (PS) of hippocampal dentate gyrus following application of low-frequency stimulation (1/30 Hz, 1.0 mA). RESULTS: Fifteen, thirty and sixty minutes after intracerebroventricular (icv) administration of an estimated final brain concentration of 2 x 10(-6) mol.L-1, (-)-7-OH-Clau caused over 30% increase relative to basal PS amplitude before administration, and 27%-41% increase relative to vehicle control (P < 0.05), while (+)-7-OH-Clau exhibited 18%-25% and 11%-20% decrease in the PS amplitude when compared with basal PS amplitude and vehicle control (P < 0.05), respectively. CONCLUSION: The basal synaptic transmission in the dentate gyrus of the rat hippocampus can be potentiated by (-)-7-OH-Clau and attenuated by (+)-7-OH-Clau, indicating that there was a stereoselective difference between the two enantiomers in the modulation of synaptic responses and plasticity.