| 肾上腺髓质素在大鼠急性脊髓损伤中的表达及其意义 |
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| 引用本文： | 郭新庆,郑启新,潘海涛. 肾上腺髓质素在大鼠急性脊髓损伤中的表达及其意义[J]. 中国现代医学杂志, 2008, 18(24): 3566-3569 |
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| 作者姓名： | 郭新庆 郑启新 潘海涛 |
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| 作者单位： | 1. 河南科技大学三附院洛阳东方医院,骨科,河南,洛阳,471003
2. 华中科技大学同济医学院附属协和医院,骨科,湖北,武汉,430022 |
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| 摘 要： | 目的 观察肾上腺髓质素(AM)在大鼠损伤脊髓中的表达及甲基强的松龙(MP)干预对AM表达的影响,并探讨脊髓损伤的保护作用机制.方法 Allen's WD法制成大鼠急性脊髓损伤模型.免疫组化观察脊髓损伤后AM表达的变化;HE染色观察脊髓损伤情况.观察MP干预对脊髓损伤后AM表达、神经功能和组织学的影响.设假手术组、脊髓损伤非MP干预组(非干预组)作为对照.结果 免疫组化各组中均有AM的表达,其中假手术组AM持续低表达;MP组及非干预组AM表达增高,8h均达峰值,以后渐低,但伤后3d仍高于假手术组(P＜0.05);MP组AM表达于伤后8 h、1 d、2 d、3 d高于非干预组(P＜0.05),3组间在伤后2 h、6d时差异无显著性(P＞0.05).形态学显示假手术组无明显异常,MP组病理变化轻于非干预组.假手术组神经功能评分均高于非干预组和MP组(P＜0.01),MP组伤后3 d,6 d的神经功能评分高于非干预组(P＜0.05).结论 AM表达增高可能是脊髓损伤自身保护机制之一.通过药物上调AM的表达可起到保护脊髓的作用,这为AM治疗脊髓损伤的临床应用提供了实验依据.
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| 关 键 词： | 肾上腺髓质素 脊髓损伤 甲基强的松龙 机制 |
Expression of adrenomedullin and its significance in acute spinal cord injury tissue of rats |
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| GUO Xin-qing , ZHENG Qi-xin , PAN Hai-tao. Expression of adrenomedullin and its significance in acute spinal cord injury tissue of rats[J]. China Journal of Modern Medicine, 2008, 18(24): 3566-3569 |
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| Authors: | GUO Xin-qing ZHENG Qi-xin PAN Hai-tao |
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| Affiliation: | GUO Xin-qing1,ZHENG Qi-xin2,PAN Hai-tao2(1.Department of Orthopedics Surgery,Dongfang Hospital,Henan University of Science , Technology,Zhengzhou,Henan,471003,P.R.China,2.Department of Orthopedics Affiliated Xiehe Hospital,Huazhong University of Science , Technology,Wuhan,Hubei,430022,P.R.China) |
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| Abstract: | [Objective] To observe the expression of adrenomedullin(AM) in the acute injured spinal cord tissues of rats and the influences on AM expression by interference of methylprednisolone(MP) and to explore the protective mechanism in acute spinal cord injury.[Methods] Acute spinal cord injury models were established by way of Allen's WD method.The expressions of AM were observed by immunohistochemical staining and the histological changes of injured spinal cord were observed by H.E staining.MP's effects on AM's... |
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| Keywords: | Adrenomedullin Spinal cord injury Methylprednisolone Mechanism |
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