幽门螺杆菌对胃上皮细胞SGC-7901凋亡的影响

目的：观察幽门螺杆菌（H. pylori）能否诱导体外培养的胃上皮细胞发生凋亡。方法：采用荧光染色、流式细胞仪和透射电子显微镜技术，观察H. pylori活菌在体外对人胃腺癌细胞系SGC-791凋亡形态与凋亡率的影响。结果：Hoechst 33258荧光染色和流式细胞术两种方法所得结果一致，3.2×10⁷ CFU/L的H. pylori组与对照组的细胞凋亡率无显著差异（P>0.05），当H. pylori的浓度1.6×10⁸ CFU/L时，随着H. pylori浓度的加大，细胞凋亡率越来越高，均与对照组有显著差异（P<0.01）；细胞110CFU/L的H. pylori共育48 h后，在透射电镜下可见到部分细胞呈现凋亡形态：核中染色质不规则凝集，核膜向多处伸出指状突起，另外还可见胞浆浓缩成均质状，可形成胞浆凋亡小体。结论：H. pylori活菌能以浓度依赖方式诱导体外培养的SGC-791发生凋亡。

Effect of Helicobacter pylori on apoptosis of SGC - 7901 in vitro

AIM: To investigate if apoptosis of gastric cancer cell line SGC-7901 could be induced by alive H. pylori in vitro. METHODS: The effect of H. pylori on cell apoptosis were estimated by Hoechst 33258 fluorochrome staining, flow cytometry and transmission electron microscope (TEM). RESULTS: Hoechst 33258 fluorochrome staining and flow cytometry presented a similar result:cell apoptosis rate of 3.2×10⁷ CFU/L and control group had no significant difference(P>0.05); when concentration of H. pylori≥1 6×10⁸ CFU/L, apoptosis rate increased in a concentration-dependent manner (P<0.01); TEM showed some apoptosis-like morphologic changes: (1) irregular accumulation of nuclear chromatin and protrusive nuclear membrane with finger-protrusion; (2) condensing cytoplasm forming apoptotic body. CONCLUSION: H. pylori could induce apoptosis of gastric epithelial cell in vitro.