神经损伤与再生中神经元GAP-43mRNA表达的原位杂交研究

目的：研究周围神经损伤过程中生长相关蛋白（GAP-43）mRNA表达的变化规律。方法：建立大鼠坐骨神经中段钳夹损伤模型，用原位杂交技术对大鼠的腰髓和背根神经节的GAP-43mRNA表达进行观察。结果：大鼠坐骨神经损伤2d后，腰髓腹角运动神经元和背根节感觉神经元可检测到GAP-43mRNA杂交信号；术后4、7和14d明显增强；术后30d减弱，60d已恢复正常。结论：周围神经损伤诱导神经元胞体GAP-43mRNA表达显著增强，表明GAP-43在神经再生过程中起重要作用。

In situ HYBRIZATION STUDY ON GAP-43mRNA EXPRESSION DURING NERVE INJURE

Objective To study GAP|43mRNA expression during nerve injure and regeneration. Methods Rat sciatic nerve was crushed then, in situ hybridization technique was used to explore GAP|43mRNA expressions in spinal cord and dorsal root ganglion (DRG). Results The neurons of spinal cord and DRG were detected to have GAP|43 hybridization sinal by 2 days after sciatic nerve lesion. At later times(4,7 and 14 days postsurgery) the anterior horn motor neurons and DRG cells showed an increase in the number of GAP|43mRNA positive neurons, followed by a significant rise in their content of GAP|43mRNA. The number of GAP|43mRNA positive neurons was decreased by 30 days postinjure, and was nearly nomal 60 days postinjure.Conclusion GAP|43mRNA expression was increased during peripheral nerve injure and regeneration. The study showed that GAP|43 may play a key role in nerve regeneration. [