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| 志贺菌属非典型1类整合子与耐多药关系 | |
| 引用本文： | 朱静媛,段广才,范清堂,郗园林.志贺菌属非典型1类整合子与耐多药关系[J].中国公共卫生,2011,27(5). |
| 作者姓名： | 朱静媛 段广才 范清堂 郗园林 |
| 作者单位： | 1. 郑州大学公共卫生学院环境卫生学教研室,河南郑,州450001 2. 郑州大学公共卫生学院流行病学教研室;河南省分子医学重点实验室(郑州大学) 3. 河南省分子医学重点实验室,郑州大学 4. 郑州大学公共卫生学院流行病学教研室 |
| 基金项目： | 卫生部科研基金(WKJ2007-2-024) |
| 摘 要： | 目的研究志贺菌属中非典型1类整合子的分布及其与耐多药性的关系。方法对实验室保存的90株志贺菌属进行8种抗生素药敏实验,针对非典型1类整合子的耐药基因盒区域设计引物进行聚合酶链反应(PCR)扩增和限制性片段长度多态性(RFLP)分析,对代表性菌株进行测序分析。结果 90株志贺菌属中有86株(95.6%)对≥3种抗生素耐药;有66株(73.3%)对氨苄青霉素(AMP)、四环素(TET)和氯霉素(CHL)联合耐药;非典型1类整合子分布于65株(72.2%)志贺菌属中;RFLP分析结果表明,所有酶切产物的带型均一,测序分析发现序列为blaoxa-30-aadA1;非典型1类整合子阳性菌株对AMP-TET-CHL联合耐药率(81.5%)高于阴性菌株(52.0%),经双侧χ2检验发现差异有统计学意义(P<0.05)。结论志贺菌属非典型1类整合子阳性与其对AMP-TET-CHL联合耐药有关,可能参与耐多药性的形成。 |
| 关 键 词： | 志贺菌属 非典型1类整合子 耐多药 |
Atypical class 1 integron and multi-drug resistance in Shigella spp. |
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| ZHU Jing-yuan,DUAN Guang-cai,FAN Qing-tang,et al..Atypical class 1 integron and multi-drug resistance in Shigella spp.[J].Chinese Journal of Public Health,2011,27(5). | |
| Authors: | ZHU Jing-yuan DUAN Guang-cai FAN Qing-tang |
| Institution: | ZHU Jing-yuan,DUAN Guang-cai,FAN Qing-tang,et al.Department of Environmental Health,College of Public Health,Zhengzhou University(Zhengzhou 450001,China) |
| Abstract: | Objective To investigate the distribution of atypical class 1 integron and its relationship to multi-drug resistance in Shigella spp.Methods Susceptibility test of 8 antimicrobials was determined in 90 Shigella strains.PCR and restriction fragment length polymorphism(RFLP) analysis of atypical class 1 integron were applied to all strains.PCR production of representative isolate was selected for sequencing analysis.Results High prevalence of multi-drug resistance(86/90,95.6%) was found.Of all isolates,66(73.... |
| Keywords: | Shigella atypical class 1 integron multi-drug resistance |
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