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| 苯并[a]芘对大鼠学习记忆及海马神经元影响 | |
| 引用本文： | 陈承志,汤艳,蒋学君,涂白杰.苯并[a]芘对大鼠学习记忆及海马神经元影响[J].中国公共卫生,2011,27(5). |
| 作者姓名： | 陈承志 汤艳 蒋学君 涂白杰 |
| 作者单位： | 1. 重庆医科大学公共卫生学院劳动卫生与环境卫生教研室,重庆,400016 2. 泸州医学院公共卫生系劳动卫生与环境卫生教研室 3. 四川大学华西公共卫生学院环境卫生教研室 |
| 基金项目： | 国家自然科学资金(30671744) |
| 摘 要： | 目的探讨苯并a]芘(BaP)对大鼠学习记忆及海马神经元的影响。方法将50只SD大鼠随机分为空白对照组、溶剂对照组、BaP低(2.5 mg/kg)、中(5 mg/kg)、高(10 mg/kg)剂量组,腹腔注射染毒,每天1次,持续30d;用Morris水迷宫测试学习记忆,电镜观察海马神经元超微结构。结果空白对照组、溶剂对照组和BaP低、中、高剂量组穿越平台次数分别为(12.03±2.43)、(11.35±2.68)、(9.75±1.78)、(7.63±1.67)、(4.38±1.46)次,在平台象限停留时间分别为(46.34±11.56)、(48.12±13.22)、(32.35±7.68)、(26.13±5.34)、(16.14±3.15)s,差异均有统计学意义(P<0.05);随染毒剂量的增加,BaP低、中、高剂量组大鼠的平均逃避潜伏期和第1次寻找平台的潜伏期均延长,平均有效策略百分比、穿越平台的次数均减少,在平台象限停留时间均缩短,差异均有统计学意义(P<0.05);电镜观察结果显示,BaP低剂量组海马神经元胞体浓缩变性,核膜皱缩变形;中剂量组出现线粒体肿胀;高剂量组出现高尔基扩张。结论 BaP亚慢性染毒... |
| 关 键 词： | 苯并[a]芘 学习记忆能力 Morris水迷宫 海马 |
Morphology in hippocampal neurons and behavioral change in rats with sub-chronic exposure of benzo(a) pyrene |
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| CHEN Cheng-zhi,TANG Yan,JIANG Xue-jun,et al..Morphology in hippocampal neurons and behavioral change in rats with sub-chronic exposure of benzo(a) pyrene[J].Chinese Journal of Public Health,2011,27(5). | |
| Authors: | CHEN Cheng-zhi TANG Yan JIANG Xue-jun |
| Institution: | CHEN Cheng-zhi,TANG Yan,JIANG Xue-jun,et al.Department of Occupational and Environmental Health,School of Public Health,Chongqing Medical University(Chongqing 400016,China) |
| Abstract: | Objective To explore the effects of benzo(a)pyrene(BaP) exposure on spatial learning and memory abilities and ultramicrostructure of hippocampal neurons in rats.Methods Fifty healthy male SD rats were randomly divided into a control group,a solvent control group and three BaP-treated groups.The exposure groups were treated by intraperitoneal injection with 2.5,5,and 10 mg/kg BaP once a day,respectively.The rats in solvent control group received an equal volume of corn oil.The treatment was lasted for 30 day... |
| Keywords: | BaP spatial learning and memory Morris water maze hippocampus |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |