| 补阳还五汤对脑缺血再灌注沙鼠认知功能影响 |
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| 引用本文： | 刘海娟,赵雅宁,李建民,马素慧,陈长香. 补阳还五汤对脑缺血再灌注沙鼠认知功能影响[J]. 中国公共卫生, 2011, 27(5) |
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| 作者姓名： | 刘海娟 赵雅宁 李建民 马素慧 陈长香 |
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| 作者单位： | 华北煤炭医学院,河北唐山,063000 |
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| 基金项目： | 河北省科技厅基金(2009276103D-3) |
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| 摘 要： | 目的探讨补阳还五汤对脑缺血再灌注保护作用及可能机制。方法 188只雄性蒙古沙鼠随机分成对照组、模型组、补阳还五汤高、低剂量组,根据Kirino方法制作沙鼠前脑缺血模型;免疫组化和免疫印迹法检测海马区磷酸化p38有丝分裂原活化蛋白激酶(MAPK)表达,原位缺口末端标记法(TUNEL)检测凋亡细胞,八臂迷宫测试法测试动物学习记忆功能。结果沙鼠脑缺血1、6 h,1、3 d后,海马磷酸化p38 MAPK表达水平分别为(1.85±0.86)、(4.40±1.26)、(6.70±1.87)、(5.67±1.58),与对照组比较,明显增高(P<0.05);脑缺血6 h,1、3、7 d后,凋亡神经细胞数分别为(9.60±2.69)、(12.68±2.99)、(16.94±3.92)、(25.82±4.61)个/视野,明显高于对照组(P<0.05);八臂迷宫结果显示,动物总错误次数、重复错误次数增多;高剂量补阳还五汤组磷酸p38 MAPK蛋白表达量及神经细胞凋亡数量与模型组比较明显下降(P<0.05);动物学习记忆功能指标总错误次数、重复错误次数明显低于模型组(P<0.05)。结论补阳还五汤可改善脑缺血所致认知功能损伤,与抑制...
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| 关 键 词： | 补阳还五汤 缺血再灌注 磷酸化p38MAPK 认知功能 |
Effect of Buyanghuanwu recipe on cognitive function of gerbils with cerebral ischemia/reperfusion injury |
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| LIU Hai-juan,ZHAO Ya-ning,LI Jian-min,et al.. Effect of Buyanghuanwu recipe on cognitive function of gerbils with cerebral ischemia/reperfusion injury[J]. Chinese Journal of Public Health, 2011, 27(5) |
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| Authors: | LIU Hai-juan ZHAO Ya-ning LI Jian-min et al. |
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| Affiliation: | LIU Hai-juan,ZHAO Ya-ning,LI Jian-min,et al.Northern China Coal Medical College(Tangshan 063000,China) |
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| Abstract: | Objective To study the effect of Buyanghuanwu recipe on cerebral ischemia/ reperfusion and its underlying mechanism in gerbils.Methods Male gerbils(n=188) were divided randomly into four groups:control group,model group,high and low dose of Buyanghuanwu recipe treatment group.Transient forebrain ischemia model was established with bilateral common carotid artery occlusion according to Kirino's method.The p38 mitogen-activated protein kinase(MAPK) phosphorylation expression was detected with immunohistochemi... |
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| Keywords: | Buyanghuanwu recipe ischemia-reperfusion phosphorylated p38 MAPK cognitive function |
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